Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS13190

Experiment: 24hr PHYTO11 MOI 0.1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS13185 and BPHYT_RS13190 are separated by 55 nucleotidesBPHYT_RS13190 and BPHYT_RS13195 are separated by 364 nucleotidesBPHYT_RS13195 and BPHYT_RS13200 are separated by 117 nucleotides BPHYT_RS13185: BPHYT_RS13185 - 2,4-dienoyl-CoA reductase, at 3,008,469 to 3,010,517 _RS13185 BPHYT_RS13190: BPHYT_RS13190 - membrane protein, at 3,010,573 to 3,011,502 _RS13190 BPHYT_RS13195: BPHYT_RS13195 - hypothetical protein, at 3,011,867 to 3,012,112 _RS13195 BPHYT_RS13200: BPHYT_RS13200 - molybdopterin binding oxidoreductase, at 3,012,230 to 3,013,021 _RS13200 Position (kb) 3010 3011 3012Strain fitness (log2 ratio) -1 0 1 2at 3009.933 kb on + strand, within BPHYT_RS13185at 3009.933 kb on + strand, within BPHYT_RS13185at 3010.718 kb on + strand, within BPHYT_RS13190at 3010.718 kb on + strand, within BPHYT_RS13190at 3010.808 kb on + strand, within BPHYT_RS13190at 3011.308 kb on + strand, within BPHYT_RS13190at 3011.384 kb on + strand, within BPHYT_RS13190at 3011.731 kb on + strandat 3011.732 kb on - strandat 3012.153 kb on - strandat 3012.454 kb on - strand, within BPHYT_RS13200at 3012.483 kb on + strand, within BPHYT_RS13200

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Per-strain Table

Position Strand Gene LocusTag Fraction 24hr PHYTO11 MOI 0.1
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3,009,933 + BPHYT_RS13185 0.71 -1.6
3,009,933 + BPHYT_RS13185 0.71 -1.0
3,010,718 + BPHYT_RS13190 0.16 -0.8
3,010,718 + BPHYT_RS13190 0.16 +0.6
3,010,808 + BPHYT_RS13190 0.25 -0.7
3,011,308 + BPHYT_RS13190 0.79 +0.1
3,011,384 + BPHYT_RS13190 0.87 -0.2
3,011,731 + -0.5
3,011,732 - -1.0
3,012,153 - -1.5
3,012,454 - BPHYT_RS13200 0.28 +1.5
3,012,483 + BPHYT_RS13200 0.32 +2.2

Or see this region's nucleotide sequence