Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS10820

Experiment: 24hr PHYTO11 MOI 0.1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS10810 and BPHYT_RS10815 are separated by 45 nucleotidesBPHYT_RS10815 and BPHYT_RS10820 are separated by 3 nucleotidesBPHYT_RS10820 and BPHYT_RS10825 overlap by 8 nucleotidesBPHYT_RS10825 and BPHYT_RS10830 are separated by 23 nucleotides BPHYT_RS10810: BPHYT_RS10810 - glucose-methanol-choline oxidoreductase, at 2,457,055 to 2,458,788 _RS10810 BPHYT_RS10815: BPHYT_RS10815 - crotonase, at 2,458,834 to 2,459,640 _RS10815 BPHYT_RS10820: BPHYT_RS10820 - 4-chlorobenzoate--CoA ligase, at 2,459,644 to 2,461,221 _RS10820 BPHYT_RS10825: BPHYT_RS10825 - (2Fe-2S)-binding protein, at 2,461,214 to 2,461,744 _RS10825 BPHYT_RS10830: BPHYT_RS10830 - carbon monoxide dehydrogenase, at 2,461,768 to 2,462,589 _RS10830 Position (kb) 2459 2460 2461 2462Strain fitness (log2 ratio) -2 -1 0 1 2at 2458.773 kb on - strandat 2458.804 kb on - strandat 2458.804 kb on - strandat 2458.919 kb on + strand, within BPHYT_RS10815at 2458.919 kb on + strand, within BPHYT_RS10815at 2459.756 kb on - strandat 2459.774 kb on + strandat 2460.017 kb on + strand, within BPHYT_RS10820at 2460.018 kb on - strand, within BPHYT_RS10820at 2460.288 kb on - strand, within BPHYT_RS10820at 2460.306 kb on - strand, within BPHYT_RS10820at 2460.409 kb on - strand, within BPHYT_RS10820at 2460.449 kb on + strand, within BPHYT_RS10820at 2460.593 kb on + strand, within BPHYT_RS10820at 2460.725 kb on + strand, within BPHYT_RS10820at 2460.725 kb on + strand, within BPHYT_RS10820at 2461.134 kb on - strandat 2461.736 kb on + strandat 2461.737 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 24hr PHYTO11 MOI 0.1
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2,458,773 - -0.8
2,458,804 - +2.3
2,458,804 - -2.3
2,458,919 + BPHYT_RS10815 0.11 +0.7
2,458,919 + BPHYT_RS10815 0.11 -1.4
2,459,756 - -0.3
2,459,774 + +0.4
2,460,017 + BPHYT_RS10820 0.24 -0.3
2,460,018 - BPHYT_RS10820 0.24 +0.4
2,460,288 - BPHYT_RS10820 0.41 +0.6
2,460,306 - BPHYT_RS10820 0.42 -1.0
2,460,409 - BPHYT_RS10820 0.48 -2.1
2,460,449 + BPHYT_RS10820 0.51 -1.2
2,460,593 + BPHYT_RS10820 0.60 +0.2
2,460,725 + BPHYT_RS10820 0.69 +0.5
2,460,725 + BPHYT_RS10820 0.69 -0.6
2,461,134 - -0.0
2,461,736 + -0.3
2,461,737 - +0.5

Or see this region's nucleotide sequence