Experiment: 24hr PHYTO11 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS10330 and BPHYT_RS10335 are separated by 27 nucleotides BPHYT_RS10335 and BPHYT_RS10340 are separated by 424 nucleotides BPHYT_RS10340 and BPHYT_RS10345 are separated by 37 nucleotides BPHYT_RS10345 and BPHYT_RS10350 are separated by 23 nucleotides
BPHYT_RS10330: BPHYT_RS10330 - NUDIX hydrolase, at 2,348,634 to 2,349,062
_RS10330
BPHYT_RS10335: BPHYT_RS10335 - hypothetical protein, at 2,349,090 to 2,349,533
_RS10335
BPHYT_RS10340: BPHYT_RS10340 - sensor protein Chase2, at 2,349,958 to 2,351,352
_RS10340
BPHYT_RS10345: BPHYT_RS10345 - flagellar motor protein MotB, at 2,351,390 to 2,351,977
_RS10345
BPHYT_RS10350: BPHYT_RS10350 - hypothetical protein, at 2,352,001 to 2,352,468
_RS10350
Position (kb)
2349
2350
2351
2352 Strain fitness (log2 ratio)
-1
0
1
2 at 2349.516 kb on - strand at 2349.672 kb on - strand at 2349.795 kb on - strand at 2349.911 kb on - strand at 2350.415 kb on + strand, within BPHYT_RS10340 at 2350.624 kb on - strand, within BPHYT_RS10340 at 2350.713 kb on - strand, within BPHYT_RS10340 at 2350.713 kb on - strand, within BPHYT_RS10340 at 2350.967 kb on + strand, within BPHYT_RS10340 at 2351.829 kb on + strand, within BPHYT_RS10345 at 2352.183 kb on + strand, within BPHYT_RS10350 at 2352.183 kb on + strand, within BPHYT_RS10350 at 2352.303 kb on - strand, within BPHYT_RS10350
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO11 MOI 0.1 remove 2,349,516 - +0.5 2,349,672 - -0.9 2,349,795 - +0.4 2,349,911 - -1.6 2,350,415 + BPHYT_RS10340 0.33 +1.8 2,350,624 - BPHYT_RS10340 0.48 +0.5 2,350,713 - BPHYT_RS10340 0.54 -0.4 2,350,713 - BPHYT_RS10340 0.54 +1.8 2,350,967 + BPHYT_RS10340 0.72 +0.3 2,351,829 + BPHYT_RS10345 0.75 -0.1 2,352,183 + BPHYT_RS10350 0.39 -0.3 2,352,183 + BPHYT_RS10350 0.39 +0.2 2,352,303 - BPHYT_RS10350 0.65 +1.0
Or see this region's nucleotide sequence