Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS07460

Experiment: 24hr PHYTO11 MOI 0.1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS07450 and BPHYT_RS07455 overlap by 4 nucleotidesBPHYT_RS07455 and BPHYT_RS07460 are separated by 23 nucleotidesBPHYT_RS07460 and BPHYT_RS07465 are separated by 441 nucleotides BPHYT_RS07450: BPHYT_RS07450 - histidine kinase, at 1,693,109 to 1,694,524 _RS07450 BPHYT_RS07455: BPHYT_RS07455 - transcriptional regulator, at 1,694,521 to 1,695,204 _RS07455 BPHYT_RS07460: BPHYT_RS07460 - hypothetical protein, at 1,695,228 to 1,695,581 _RS07460 BPHYT_RS07465: BPHYT_RS07465 - FAD-dependent pyridine nucleotide-disulfide oxidoreductase, at 1,696,023 to 1,697,360 _RS07465 Position (kb) 1695 1696Strain fitness (log2 ratio) -1 0 1 2 3at 1694.281 kb on - strand, within BPHYT_RS07450at 1695.162 kb on - strandat 1695.614 kb on + strandat 1695.648 kb on + strandat 1696.020 kb on + strandat 1696.126 kb on + strandat 1696.139 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 24hr PHYTO11 MOI 0.1
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1,694,281 - BPHYT_RS07450 0.83 -1.5
1,695,162 - +1.7
1,695,614 + +0.0
1,695,648 + +1.3
1,696,020 + +1.5
1,696,126 + +0.3
1,696,139 - +2.8

Or see this region's nucleotide sequence