Experiment: 24hr PHYTO11 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS32185 and BPHYT_RS32190 are separated by 1 nucleotides BPHYT_RS32190 and BPHYT_RS32195 are separated by 16 nucleotides BPHYT_RS32195 and BPHYT_RS32200 are separated by 41 nucleotides BPHYT_RS32200 and BPHYT_RS32205 are separated by 290 nucleotides BPHYT_RS32205 and BPHYT_RS32210 are separated by 377 nucleotides
BPHYT_RS32185: BPHYT_RS32185 - type IV secretion protein VirB4, at 2,793,237 to 2,795,702
_RS32185
BPHYT_RS32190: BPHYT_RS32190 - conjugal transfer protein TraD, at 2,795,704 to 2,796,048
_RS32190
BPHYT_RS32195: BPHYT_RS32195 - type VI secretion protein, at 2,796,065 to 2,796,406
_RS32195
BPHYT_RS32200: BPHYT_RS32200 - lytic transglycosylase, at 2,796,448 to 2,797,092
_RS32200
BPHYT_RS32205: BPHYT_RS32205 - hypothetical protein, at 2,797,383 to 2,797,622
_RS32205
BPHYT_RS32210: BPHYT_RS32210 - hypothetical protein, at 2,798,000 to 2,798,284
_RS32210
Position (kb)
2796
2797
2798 Strain fitness (log2 ratio)
-2
-1
0
1 at 2795.587 kb on + strand at 2795.798 kb on + strand, within BPHYT_RS32190 at 2796.021 kb on + strand at 2796.025 kb on - strand at 2796.025 kb on - strand at 2796.601 kb on + strand, within BPHYT_RS32200 at 2796.657 kb on - strand, within BPHYT_RS32200 at 2797.960 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO11 MOI 1 remove 2,795,587 + +0.2 2,795,798 + BPHYT_RS32190 0.27 +1.6 2,796,021 + +1.0 2,796,025 - -0.8 2,796,025 - -2.3 2,796,601 + BPHYT_RS32200 0.24 +0.3 2,796,657 - BPHYT_RS32200 0.32 +1.5 2,797,960 - -0.8
Or see this region's nucleotide sequence