Experiment: 24hr PHYTO11 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS28355 and BPHYT_RS28360 are separated by 111 nucleotides BPHYT_RS28360 and BPHYT_RS28365 are separated by 15 nucleotides BPHYT_RS28365 and BPHYT_RS28370 are separated by 32 nucleotides BPHYT_RS28370 and BPHYT_RS28375 are separated by 506 nucleotides
BPHYT_RS28355: BPHYT_RS28355 - LysR family transcriptional regulator, at 1,914,232 to 1,915,134
_RS28355
BPHYT_RS28360: BPHYT_RS28360 - carboxymuconolactone decarboxylase, at 1,915,246 to 1,915,569
_RS28360
BPHYT_RS28365: BPHYT_RS28365 - Germin subfamily 1 member 15, at 1,915,585 to 1,915,989
_RS28365
BPHYT_RS28370: BPHYT_RS28370 - peroxidase, at 1,916,022 to 1,916,885
_RS28370
BPHYT_RS28375: BPHYT_RS28375 - hypothetical protein, at 1,917,392 to 1,917,952
_RS28375
Position (kb)
1916
1917 Strain fitness (log2 ratio)
-2
-1
0
1 at 1915.052 kb on - strand at 1915.072 kb on - strand at 1915.136 kb on + strand at 1915.241 kb on - strand at 1915.243 kb on + strand at 1915.501 kb on + strand, within BPHYT_RS28360 at 1915.502 kb on - strand, within BPHYT_RS28360 at 1915.860 kb on + strand, within BPHYT_RS28365 at 1915.969 kb on + strand at 1915.970 kb on - strand at 1916.191 kb on + strand, within BPHYT_RS28370 at 1917.252 kb on - strand at 1917.783 kb on + strand, within BPHYT_RS28375
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO11 MOI 1 remove 1,915,052 - -0.1 1,915,072 - -0.1 1,915,136 + +0.4 1,915,241 - +0.5 1,915,243 + +1.3 1,915,501 + BPHYT_RS28360 0.79 -1.4 1,915,502 - BPHYT_RS28360 0.79 -1.8 1,915,860 + BPHYT_RS28365 0.68 -0.5 1,915,969 + +0.7 1,915,970 - +0.3 1,916,191 + BPHYT_RS28370 0.20 -2.7 1,917,252 - +0.1 1,917,783 + BPHYT_RS28375 0.70 -0.1
Or see this region's nucleotide sequence