Experiment: 24hr PHYTO11 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS25465 and BPHYT_RS25470 are separated by 26 nucleotides BPHYT_RS25470 and BPHYT_RS25475 are separated by 115 nucleotides BPHYT_RS25475 and BPHYT_RS25480 are separated by 173 nucleotides BPHYT_RS25480 and BPHYT_RS25485 are separated by 91 nucleotides
BPHYT_RS25465: BPHYT_RS25465 - 1,2-oxophytodienoate reductase, at 1,291,383 to 1,292,471
_RS25465
BPHYT_RS25470: BPHYT_RS25470 - alkylhydroperoxidase, at 1,292,498 to 1,293,034
_RS25470
BPHYT_RS25475: BPHYT_RS25475 - LysR family transcriptional regulator, at 1,293,150 to 1,294,064
_RS25475
BPHYT_RS25480: BPHYT_RS25480 - hypothetical protein, at 1,294,238 to 1,294,879
_RS25480
BPHYT_RS25485: BPHYT_RS25485 - hypothetical protein, at 1,294,971 to 1,295,750
_RS25485
Position (kb) 1293
1294
1295 Strain fitness (log2 ratio) -2
-1
0
1 at 1292.208 kb on + strand, within BPHYT_RS25465 at 1292.342 kb on + strand, within BPHYT_RS25465 at 1292.476 kb on - strand at 1292.710 kb on + strand, within BPHYT_RS25470 at 1292.711 kb on - strand, within BPHYT_RS25470 at 1293.099 kb on - strand at 1293.099 kb on - strand at 1293.139 kb on + strand at 1293.293 kb on - strand, within BPHYT_RS25475 at 1294.151 kb on + strand at 1294.353 kb on + strand, within BPHYT_RS25480 at 1294.355 kb on + strand, within BPHYT_RS25480 at 1294.508 kb on - strand, within BPHYT_RS25480 at 1294.511 kb on + strand, within BPHYT_RS25480 at 1294.512 kb on - strand, within BPHYT_RS25480
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO11 MOI 1 remove 1,292,208 + BPHYT_RS25465 0.76 -0.3 1,292,342 + BPHYT_RS25465 0.88 +1.3 1,292,476 - -0.9 1,292,710 + BPHYT_RS25470 0.39 +0.0 1,292,711 - BPHYT_RS25470 0.40 +0.1 1,293,099 - +1.1 1,293,099 - -2.0 1,293,139 + -1.1 1,293,293 - BPHYT_RS25475 0.16 -1.2 1,294,151 + +1.4 1,294,353 + BPHYT_RS25480 0.18 -0.7 1,294,355 + BPHYT_RS25480 0.18 -1.9 1,294,508 - BPHYT_RS25480 0.42 -2.1 1,294,511 + BPHYT_RS25480 0.43 -1.6 1,294,512 - BPHYT_RS25480 0.43 +1.1
Or see this region's nucleotide sequence