Experiment: 24hr PHYTO11 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS25460 and BPHYT_RS25465 are separated by 385 nucleotides BPHYT_RS25465 and BPHYT_RS25470 are separated by 26 nucleotides BPHYT_RS25470 and BPHYT_RS25475 are separated by 115 nucleotides
BPHYT_RS25460: BPHYT_RS25460 - LysR family transcriptional regulator, at 1,290,101 to 1,290,997
_RS25460
BPHYT_RS25465: BPHYT_RS25465 - 1,2-oxophytodienoate reductase, at 1,291,383 to 1,292,471
_RS25465
BPHYT_RS25470: BPHYT_RS25470 - alkylhydroperoxidase, at 1,292,498 to 1,293,034
_RS25470
BPHYT_RS25475: BPHYT_RS25475 - LysR family transcriptional regulator, at 1,293,150 to 1,294,064
_RS25475
Position (kb) 1291
1292
1293 Strain fitness (log2 ratio) -2
-1
0
1 at 1291.300 kb on + strand at 1291.409 kb on - strand at 1291.416 kb on - strand at 1291.526 kb on - strand, within BPHYT_RS25465 at 1291.821 kb on + strand, within BPHYT_RS25465 at 1292.059 kb on - strand, within BPHYT_RS25465 at 1292.208 kb on + strand, within BPHYT_RS25465 at 1292.342 kb on + strand, within BPHYT_RS25465 at 1292.476 kb on - strand at 1292.710 kb on + strand, within BPHYT_RS25470 at 1292.711 kb on - strand, within BPHYT_RS25470 at 1293.099 kb on - strand at 1293.099 kb on - strand at 1293.139 kb on + strand at 1293.293 kb on - strand, within BPHYT_RS25475
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO11 MOI 1 remove 1,291,300 + -1.6 1,291,409 - -0.4 1,291,416 - +0.4 1,291,526 - BPHYT_RS25465 0.13 -0.7 1,291,821 + BPHYT_RS25465 0.40 +0.1 1,292,059 - BPHYT_RS25465 0.62 -1.3 1,292,208 + BPHYT_RS25465 0.76 -0.3 1,292,342 + BPHYT_RS25465 0.88 +1.3 1,292,476 - -0.9 1,292,710 + BPHYT_RS25470 0.39 +0.0 1,292,711 - BPHYT_RS25470 0.40 +0.1 1,293,099 - +1.1 1,293,099 - -2.0 1,293,139 + -1.1 1,293,293 - BPHYT_RS25475 0.16 -1.2
Or see this region's nucleotide sequence