Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS23925

Experiment: 24hr PHYTO11 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS23915 and BPHYT_RS23920 overlap by 4 nucleotidesBPHYT_RS23920 and BPHYT_RS23925 overlap by 4 nucleotidesBPHYT_RS23925 and BPHYT_RS23930 overlap by 13 nucleotidesBPHYT_RS23930 and BPHYT_RS23935 are separated by 2 nucleotides BPHYT_RS23915: BPHYT_RS23915 - malonate decarboxylase subunit gamma, at 931,399 to 932,217 _RS23915 BPHYT_RS23920: BPHYT_RS23920 - malonate decarboxylase subunit beta, at 932,214 to 933,098 _RS23920 BPHYT_RS23925: BPHYT_RS23925 - malonate decarboxylase subunit delta, at 933,095 to 933,409 _RS23925 BPHYT_RS23930: BPHYT_RS23930 - triphosphoribosyl-dephospho-CoA synthase, at 933,397 to 934,320 _RS23930 BPHYT_RS23935: BPHYT_RS23935 - malonate decarboxylase subunit alpha, at 934,323 to 936,005 _RS23935 Position (kb) 933 934Strain fitness (log2 ratio) -2 -1 0 1at 932.572 kb on + strand, within BPHYT_RS23920at 932.573 kb on - strand, within BPHYT_RS23920at 933.093 kb on - strandat 934.128 kb on + strand, within BPHYT_RS23930at 934.128 kb on + strand, within BPHYT_RS23930at 934.355 kb on - strandat 934.355 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 24hr PHYTO11 MOI 1
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932,572 + BPHYT_RS23920 0.40 -1.6
932,573 - BPHYT_RS23920 0.41 -1.5
933,093 - +0.0
934,128 + BPHYT_RS23930 0.79 +0.8
934,128 + BPHYT_RS23930 0.79 +0.1
934,355 - -2.2
934,355 - -1.7

Or see this region's nucleotide sequence