Experiment: 24hr PHYTO11 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS21610 and BPHYT_RS21615 are separated by 117 nucleotides BPHYT_RS21615 and BPHYT_RS21620 overlap by 4 nucleotides BPHYT_RS21620 and BPHYT_RS21625 are separated by 117 nucleotides
BPHYT_RS21610: BPHYT_RS21610 - DNA-binding protein, at 404,332 to 405,861
_RS21610
BPHYT_RS21615: BPHYT_RS21615 - FMN-dependent NADH-azoreductase, at 405,979 to 406,653
_RS21615
BPHYT_RS21620: BPHYT_RS21620 - GCN5 family acetyltransferase, at 406,650 to 407,117
_RS21620
BPHYT_RS21625: BPHYT_RS21625 - LysR family transcriptional regulator, at 407,235 to 408,149
_RS21625
Position (kb) 405
406
407 Strain fitness (log2 ratio) -2
-1
0
1 at 405.349 kb on + strand, within BPHYT_RS21610 at 405.397 kb on + strand, within BPHYT_RS21610 at 405.548 kb on - strand, within BPHYT_RS21610 at 405.548 kb on - strand, within BPHYT_RS21610 at 405.548 kb on - strand, within BPHYT_RS21610 at 405.563 kb on + strand, within BPHYT_RS21610 at 405.872 kb on - strand at 405.872 kb on - strand at 405.997 kb on - strand at 405.997 kb on - strand at 406.184 kb on + strand, within BPHYT_RS21615 at 406.735 kb on + strand, within BPHYT_RS21620 at 407.320 kb on + strand at 407.321 kb on - strand at 407.623 kb on + strand, within BPHYT_RS21625
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO11 MOI 1 remove 405,349 + BPHYT_RS21610 0.66 -0.6 405,397 + BPHYT_RS21610 0.70 -0.5 405,548 - BPHYT_RS21610 0.79 -1.5 405,548 - BPHYT_RS21610 0.79 +0.2 405,548 - BPHYT_RS21610 0.79 +0.5 405,563 + BPHYT_RS21610 0.80 +0.8 405,872 - +0.4 405,872 - +0.3 405,997 - +0.6 405,997 - -1.1 406,184 + BPHYT_RS21615 0.30 -0.6 406,735 + BPHYT_RS21620 0.18 -1.4 407,320 + -1.8 407,321 - -0.8 407,623 + BPHYT_RS21625 0.42 -0.8
Or see this region's nucleotide sequence