Experiment: 24hr PHYTO11 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS20795 and BPHYT_RS20800 are separated by 95 nucleotides BPHYT_RS20800 and BPHYT_RS20805 are separated by 126 nucleotides
BPHYT_RS20795: BPHYT_RS20795 - sorbosone dehydrogenase, at 227,041 to 228,549
_RS20795
BPHYT_RS20800: BPHYT_RS20800 - alanine-phosphoribitol ligase, at 228,645 to 230,318
_RS20800
BPHYT_RS20805: BPHYT_RS20805 - LacI family transcriptional regulator, at 230,445 to 231,524
_RS20805
Position (kb) 228
229
230
231 Strain fitness (log2 ratio) -2
-1
0
1 at 227.742 kb on + strand, within BPHYT_RS20795 at 227.742 kb on + strand, within BPHYT_RS20795 at 227.743 kb on - strand, within BPHYT_RS20795 at 228.270 kb on + strand, within BPHYT_RS20795 at 228.491 kb on - strand at 228.491 kb on - strand at 228.622 kb on - strand at 228.648 kb on + strand at 228.648 kb on + strand at 228.648 kb on + strand at 228.648 kb on + strand at 228.654 kb on + strand at 228.654 kb on + strand at 228.765 kb on + strand at 228.767 kb on + strand at 228.943 kb on - strand, within BPHYT_RS20800 at 229.143 kb on + strand, within BPHYT_RS20800 at 229.143 kb on + strand, within BPHYT_RS20800 at 229.565 kb on + strand, within BPHYT_RS20800 at 229.565 kb on + strand, within BPHYT_RS20800 at 229.565 kb on + strand, within BPHYT_RS20800 at 229.791 kb on + strand, within BPHYT_RS20800 at 230.020 kb on - strand, within BPHYT_RS20800 at 230.369 kb on - strand at 230.817 kb on + strand, within BPHYT_RS20805 at 230.817 kb on + strand, within BPHYT_RS20805 at 230.818 kb on - strand, within BPHYT_RS20805
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO11 MOI 1 remove 227,742 + BPHYT_RS20795 0.46 +0.3 227,742 + BPHYT_RS20795 0.46 -1.8 227,743 - BPHYT_RS20795 0.47 -1.6 228,270 + BPHYT_RS20795 0.81 -1.9 228,491 - +0.7 228,491 - +0.1 228,622 - +0.8 228,648 + +0.5 228,648 + -0.8 228,648 + +0.2 228,648 + +0.1 228,654 + +0.9 228,654 + +1.3 228,765 + +0.1 228,767 + +0.3 228,943 - BPHYT_RS20800 0.18 -0.3 229,143 + BPHYT_RS20800 0.30 +0.6 229,143 + BPHYT_RS20800 0.30 +0.4 229,565 + BPHYT_RS20800 0.55 -1.1 229,565 + BPHYT_RS20800 0.55 -1.0 229,565 + BPHYT_RS20800 0.55 +0.7 229,791 + BPHYT_RS20800 0.68 +0.5 230,020 - BPHYT_RS20800 0.82 -0.0 230,369 - +0.4 230,817 + BPHYT_RS20805 0.34 -0.9 230,817 + BPHYT_RS20805 0.34 -1.7 230,818 - BPHYT_RS20805 0.35 +0.3
Or see this region's nucleotide sequence