Experiment: 24hr PHYTO11 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS20050 and BPHYT_RS20055 are separated by 342 nucleotides BPHYT_RS20055 and BPHYT_RS20060 are separated by 227 nucleotides BPHYT_RS20060 and BPHYT_RS20065 overlap by 1 nucleotides BPHYT_RS20065 and BPHYT_RS20070 are separated by 81 nucleotides
BPHYT_RS20050: BPHYT_RS20050 - hypothetical protein, at 43,294 to 43,542
_RS20050
BPHYT_RS20055: BPHYT_RS20055 - hypothetical protein, at 43,885 to 44,202
_RS20055
BPHYT_RS20060: BPHYT_RS20060 - hypothetical protein, at 44,430 to 45,122
_RS20060
BPHYT_RS20065: BPHYT_RS20065 - hypothetical protein, at 45,122 to 45,604
_RS20065
BPHYT_RS20070: BPHYT_RS20070 - hypothetical protein, at 45,686 to 47,119
_RS20070
Position (kb) 44
45
46 Strain fitness (log2 ratio) -1
0
1 at 43.776 kb on + strand at 43.843 kb on - strand at 43.886 kb on + strand at 44.069 kb on + strand, within BPHYT_RS20055 at 44.069 kb on + strand, within BPHYT_RS20055 at 44.236 kb on + strand at 44.360 kb on - strand at 44.639 kb on - strand, within BPHYT_RS20060 at 45.145 kb on + strand at 45.418 kb on - strand, within BPHYT_RS20065 at 45.630 kb on + strand at 45.631 kb on - strand at 45.646 kb on - strand at 45.661 kb on - strand at 46.098 kb on - strand, within BPHYT_RS20070
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO11 MOI 1 remove 43,776 + +1.3 43,843 - +0.4 43,886 + -0.7 44,069 + BPHYT_RS20055 0.58 +1.3 44,069 + BPHYT_RS20055 0.58 -0.4 44,236 + +0.2 44,360 - +1.4 44,639 - BPHYT_RS20060 0.30 -1.3 45,145 + +0.6 45,418 - BPHYT_RS20065 0.61 -1.2 45,630 + +0.2 45,631 - -0.7 45,646 - -0.8 45,661 - +0.2 46,098 - BPHYT_RS20070 0.29 -1.0
Or see this region's nucleotide sequence