Experiment: 24hr PHYTO11 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS14725 and BPHYT_RS14730 overlap by 4 nucleotides BPHYT_RS14730 and BPHYT_RS14735 are separated by 1 nucleotides BPHYT_RS14735 and BPHYT_RS14740 are separated by 201 nucleotides
BPHYT_RS14725: BPHYT_RS14725 - peptidase M48, at 3,353,725 to 3,354,984
_RS14725
BPHYT_RS14730: BPHYT_RS14730 - GTPase RsgA, at 3,354,981 to 3,355,931
_RS14730
BPHYT_RS14735: BPHYT_RS14735 - hypothetical protein, at 3,355,933 to 3,356,253
_RS14735
BPHYT_RS14740: BPHYT_RS14740 - threonine-phosphate decarboxylase, at 3,356,455 to 3,357,393
_RS14740
Position (kb)
3355
3356
3357 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3355.890 kb on + strand at 3356.205 kb on + strand, within BPHYT_RS14735 at 3356.283 kb on + strand at 3356.284 kb on - strand at 3356.506 kb on + strand at 3356.507 kb on - strand at 3356.726 kb on + strand, within BPHYT_RS14740 at 3356.727 kb on - strand, within BPHYT_RS14740 at 3356.986 kb on - strand, within BPHYT_RS14740 at 3356.986 kb on - strand, within BPHYT_RS14740 at 3356.986 kb on - strand, within BPHYT_RS14740 at 3357.013 kb on - strand, within BPHYT_RS14740 at 3357.021 kb on + strand, within BPHYT_RS14740 at 3357.137 kb on + strand, within BPHYT_RS14740 at 3357.186 kb on - strand, within BPHYT_RS14740 at 3357.194 kb on + strand, within BPHYT_RS14740 at 3357.194 kb on + strand, within BPHYT_RS14740 at 3357.194 kb on + strand, within BPHYT_RS14740 at 3357.194 kb on + strand, within BPHYT_RS14740 at 3357.194 kb on + strand, within BPHYT_RS14740 at 3357.195 kb on - strand, within BPHYT_RS14740 at 3357.195 kb on - strand, within BPHYT_RS14740 at 3357.195 kb on - strand, within BPHYT_RS14740 at 3357.197 kb on + strand, within BPHYT_RS14740 at 3357.197 kb on + strand, within BPHYT_RS14740
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO11 MOI 1 remove 3,355,890 + +1.1 3,356,205 + BPHYT_RS14735 0.85 -2.0 3,356,283 + +0.7 3,356,284 - -1.7 3,356,506 + -0.0 3,356,507 - +0.8 3,356,726 + BPHYT_RS14740 0.29 +1.4 3,356,727 - BPHYT_RS14740 0.29 -0.1 3,356,986 - BPHYT_RS14740 0.57 -0.7 3,356,986 - BPHYT_RS14740 0.57 +1.1 3,356,986 - BPHYT_RS14740 0.57 +1.0 3,357,013 - BPHYT_RS14740 0.59 +1.9 3,357,021 + BPHYT_RS14740 0.60 -0.1 3,357,137 + BPHYT_RS14740 0.73 -0.5 3,357,186 - BPHYT_RS14740 0.78 -1.0 3,357,194 + BPHYT_RS14740 0.79 +1.3 3,357,194 + BPHYT_RS14740 0.79 +1.1 3,357,194 + BPHYT_RS14740 0.79 +1.6 3,357,194 + BPHYT_RS14740 0.79 +0.3 3,357,194 + BPHYT_RS14740 0.79 +0.4 3,357,195 - BPHYT_RS14740 0.79 +0.4 3,357,195 - BPHYT_RS14740 0.79 +0.2 3,357,195 - BPHYT_RS14740 0.79 -0.4 3,357,197 + BPHYT_RS14740 0.79 +0.9 3,357,197 + BPHYT_RS14740 0.79 +0.1
Or see this region's nucleotide sequence