Experiment: 24hr PHYTO11 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS10340 and BPHYT_RS10345 are separated by 37 nucleotides BPHYT_RS10345 and BPHYT_RS10350 are separated by 23 nucleotides BPHYT_RS10350 and BPHYT_RS10355 are separated by 322 nucleotides
BPHYT_RS10340: BPHYT_RS10340 - sensor protein Chase2, at 2,349,958 to 2,351,352
_RS10340
BPHYT_RS10345: BPHYT_RS10345 - flagellar motor protein MotB, at 2,351,390 to 2,351,977
_RS10345
BPHYT_RS10350: BPHYT_RS10350 - hypothetical protein, at 2,352,001 to 2,352,468
_RS10350
BPHYT_RS10355: BPHYT_RS10355 - nitrate reductase, at 2,352,791 to 2,354,248
_RS10355
Position (kb)
2351
2352 Strain fitness (log2 ratio)
-2
-1
0
1 at 2350.415 kb on + strand, within BPHYT_RS10340 at 2350.624 kb on - strand, within BPHYT_RS10340 at 2350.713 kb on - strand, within BPHYT_RS10340 at 2350.713 kb on - strand, within BPHYT_RS10340 at 2350.967 kb on + strand, within BPHYT_RS10340 at 2351.829 kb on + strand, within BPHYT_RS10345 at 2352.183 kb on + strand, within BPHYT_RS10350 at 2352.183 kb on + strand, within BPHYT_RS10350 at 2352.303 kb on - strand, within BPHYT_RS10350 at 2352.560 kb on - strand at 2352.654 kb on - strand at 2352.821 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO11 MOI 1 remove 2,350,415 + BPHYT_RS10340 0.33 +0.5 2,350,624 - BPHYT_RS10340 0.48 -0.4 2,350,713 - BPHYT_RS10340 0.54 -0.8 2,350,713 - BPHYT_RS10340 0.54 -0.0 2,350,967 + BPHYT_RS10340 0.72 -2.0 2,351,829 + BPHYT_RS10345 0.75 -1.5 2,352,183 + BPHYT_RS10350 0.39 +1.0 2,352,183 + BPHYT_RS10350 0.39 +0.4 2,352,303 - BPHYT_RS10350 0.65 +0.2 2,352,560 - -0.5 2,352,654 - -1.5 2,352,821 + +0.3
Or see this region's nucleotide sequence