Experiment: 24hr PHYTO11 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS10205 and BPHYT_RS10210 are separated by 151 nucleotides BPHYT_RS10210 and BPHYT_RS10215 are separated by 88 nucleotides BPHYT_RS10215 and BPHYT_RS10220 are separated by 166 nucleotides BPHYT_RS10220 and BPHYT_RS10225 are separated by 62 nucleotides
BPHYT_RS10205: BPHYT_RS10205 - D-lactate dehydrogenase, at 2,323,605 to 2,325,344
_RS10205
BPHYT_RS10210: BPHYT_RS10210 - LysR family transcriptional regulator, at 2,325,496 to 2,326,395
_RS10210
BPHYT_RS10215: BPHYT_RS10215 - sulfate transporter, at 2,326,484 to 2,326,954
_RS10215
BPHYT_RS10220: BPHYT_RS10220 - hypothetical protein, at 2,327,121 to 2,327,330
_RS10220
BPHYT_RS10225: BPHYT_RS10225 - GYD family protein, at 2,327,393 to 2,327,686
_RS10225
Position (kb) 2325
2326
2327 Strain fitness (log2 ratio) -2
-1
0
1 at 2324.721 kb on - strand, within BPHYT_RS10205 at 2324.814 kb on - strand, within BPHYT_RS10205 at 2325.050 kb on - strand, within BPHYT_RS10205 at 2325.050 kb on - strand, within BPHYT_RS10205 at 2325.591 kb on - strand, within BPHYT_RS10210 at 2326.696 kb on + strand, within BPHYT_RS10215 at 2327.247 kb on - strand, within BPHYT_RS10220
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO11 MOI 1 remove 2,324,721 - BPHYT_RS10205 0.64 -1.9 2,324,814 - BPHYT_RS10205 0.69 -1.5 2,325,050 - BPHYT_RS10205 0.83 +1.3 2,325,050 - BPHYT_RS10205 0.83 -0.2 2,325,591 - BPHYT_RS10210 0.11 -0.9 2,326,696 + BPHYT_RS10215 0.45 -1.0 2,327,247 - BPHYT_RS10220 0.60 -0.3
Or see this region's nucleotide sequence