Experiment: 24hr PHYTO11 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS10000 and BPHYT_RS10005 are separated by 200 nucleotides BPHYT_RS10005 and BPHYT_RS10010 are separated by 113 nucleotides BPHYT_RS10010 and BPHYT_RS10015 overlap by 4 nucleotides
BPHYT_RS10000: BPHYT_RS10000 - glucoamylase, at 2,277,862 to 2,279,733
_RS10000
BPHYT_RS10005: BPHYT_RS10005 - hypothetical protein, at 2,279,934 to 2,280,305
_RS10005
BPHYT_RS10010: BPHYT_RS10010 - cupin, at 2,280,419 to 2,280,850
_RS10010
BPHYT_RS10015: BPHYT_RS10015 - NAD(P)H dehydrogenase (quinone), at 2,280,847 to 2,281,530
_RS10015
Position (kb)
2279
2280
2281 Strain fitness (log2 ratio)
-2
-1
0
1 at 2278.966 kb on + strand, within BPHYT_RS10000 at 2279.061 kb on + strand, within BPHYT_RS10000 at 2279.504 kb on + strand, within BPHYT_RS10000 at 2279.504 kb on + strand, within BPHYT_RS10000 at 2279.504 kb on + strand, within BPHYT_RS10000 at 2279.505 kb on - strand, within BPHYT_RS10000 at 2279.505 kb on - strand, within BPHYT_RS10000 at 2280.111 kb on + strand, within BPHYT_RS10005 at 2280.111 kb on + strand, within BPHYT_RS10005 at 2280.363 kb on - strand at 2280.369 kb on - strand at 2281.059 kb on + strand, within BPHYT_RS10015 at 2281.060 kb on - strand, within BPHYT_RS10015 at 2281.079 kb on + strand, within BPHYT_RS10015 at 2281.179 kb on - strand, within BPHYT_RS10015 at 2281.245 kb on - strand, within BPHYT_RS10015 at 2281.245 kb on - strand, within BPHYT_RS10015 at 2281.245 kb on - strand, within BPHYT_RS10015
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO11 MOI 1 remove 2,278,966 + BPHYT_RS10000 0.59 +0.1 2,279,061 + BPHYT_RS10000 0.64 -0.4 2,279,504 + BPHYT_RS10000 0.88 +0.8 2,279,504 + BPHYT_RS10000 0.88 +1.2 2,279,504 + BPHYT_RS10000 0.88 -0.3 2,279,505 - BPHYT_RS10000 0.88 +0.2 2,279,505 - BPHYT_RS10000 0.88 -0.3 2,280,111 + BPHYT_RS10005 0.48 -1.3 2,280,111 + BPHYT_RS10005 0.48 -2.5 2,280,363 - -0.1 2,280,369 - +1.0 2,281,059 + BPHYT_RS10015 0.31 -2.0 2,281,060 - BPHYT_RS10015 0.31 -0.7 2,281,079 + BPHYT_RS10015 0.34 -0.6 2,281,179 - BPHYT_RS10015 0.49 -0.9 2,281,245 - BPHYT_RS10015 0.58 -0.6 2,281,245 - BPHYT_RS10015 0.58 -1.1 2,281,245 - BPHYT_RS10015 0.58 -0.2
Or see this region's nucleotide sequence