Experiment: 24hr PHYTO11 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS09790 and BPHYT_RS09795 are separated by 710 nucleotides BPHYT_RS09795 and BPHYT_RS09800 are separated by 361 nucleotides BPHYT_RS09800 and BPHYT_RS09805 are separated by 251 nucleotides
BPHYT_RS09790: BPHYT_RS09790 - acyltransferase, at 2,224,009 to 2,225,220
_RS09790
BPHYT_RS09795: BPHYT_RS09795 - UTP--glucose-1-phosphate uridylyltransferase, at 2,225,931 to 2,226,848
_RS09795
BPHYT_RS09800: BPHYT_RS09800 - hypothetical protein, at 2,227,210 to 2,227,542
_RS09800
BPHYT_RS09805: BPHYT_RS09805 - hypothetical protein, at 2,227,794 to 2,228,591
_RS09805
Position (kb)
2225
2226
2227 Strain fitness (log2 ratio)
-2
-1
0
1 at 2224.970 kb on + strand, within BPHYT_RS09790 at 2225.060 kb on + strand, within BPHYT_RS09790 at 2225.061 kb on - strand, within BPHYT_RS09790 at 2225.312 kb on + strand at 2225.313 kb on - strand at 2225.313 kb on - strand at 2225.316 kb on - strand at 2225.622 kb on - strand at 2225.832 kb on - strand at 2226.234 kb on + strand, within BPHYT_RS09795 at 2226.234 kb on + strand, within BPHYT_RS09795 at 2226.234 kb on + strand, within BPHYT_RS09795 at 2226.235 kb on - strand, within BPHYT_RS09795 at 2226.553 kb on - strand, within BPHYT_RS09795 at 2226.789 kb on + strand at 2226.790 kb on - strand at 2226.790 kb on - strand at 2226.790 kb on - strand at 2226.791 kb on + strand at 2226.930 kb on + strand at 2227.030 kb on + strand at 2227.227 kb on - strand at 2227.347 kb on + strand, within BPHYT_RS09800 at 2227.541 kb on + strand at 2227.541 kb on + strand at 2227.624 kb on + strand at 2227.668 kb on + strand at 2227.789 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO11 MOI 1 remove 2,224,970 + BPHYT_RS09790 0.79 +1.2 2,225,060 + BPHYT_RS09790 0.87 -0.9 2,225,061 - BPHYT_RS09790 0.87 -1.0 2,225,312 + +1.2 2,225,313 - +1.2 2,225,313 - -1.0 2,225,316 - -0.6 2,225,622 - +0.7 2,225,832 - -0.2 2,226,234 + BPHYT_RS09795 0.33 +0.6 2,226,234 + BPHYT_RS09795 0.33 -0.1 2,226,234 + BPHYT_RS09795 0.33 -1.0 2,226,235 - BPHYT_RS09795 0.33 -0.6 2,226,553 - BPHYT_RS09795 0.68 -0.3 2,226,789 + +0.1 2,226,790 - -0.6 2,226,790 - -1.1 2,226,790 - -0.0 2,226,791 + +0.3 2,226,930 + -0.8 2,227,030 + -0.1 2,227,227 - +0.9 2,227,347 + BPHYT_RS09800 0.41 -0.6 2,227,541 + +0.3 2,227,541 + -0.4 2,227,624 + -1.8 2,227,668 + -0.1 2,227,789 + -0.1
Or see this region's nucleotide sequence