Experiment: 24hr PHYTO11 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS09780 and BPHYT_RS09785 overlap by 4 nucleotides BPHYT_RS09785 and BPHYT_RS09790 are separated by 116 nucleotides
BPHYT_RS09780: BPHYT_RS09780 - polysaccharide biosynthesis protein, at 2,219,973 to 2,221,439
_RS09780
BPHYT_RS09785: BPHYT_RS09785 - glycosyl transferase family 1, at 2,221,436 to 2,223,892
_RS09785
BPHYT_RS09790: BPHYT_RS09790 - acyltransferase, at 2,224,009 to 2,225,220
_RS09790
Position (kb)
2221
2222
2223
2224 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2220.438 kb on + strand, within BPHYT_RS09780 at 2220.750 kb on + strand, within BPHYT_RS09780 at 2221.599 kb on - strand at 2221.764 kb on - strand, within BPHYT_RS09785 at 2221.764 kb on - strand, within BPHYT_RS09785 at 2221.811 kb on + strand, within BPHYT_RS09785 at 2221.886 kb on + strand, within BPHYT_RS09785 at 2221.887 kb on - strand, within BPHYT_RS09785 at 2221.932 kb on - strand, within BPHYT_RS09785 at 2222.084 kb on + strand, within BPHYT_RS09785 at 2222.084 kb on + strand, within BPHYT_RS09785 at 2222.191 kb on + strand, within BPHYT_RS09785 at 2222.693 kb on + strand, within BPHYT_RS09785 at 2222.704 kb on + strand, within BPHYT_RS09785 at 2223.467 kb on + strand, within BPHYT_RS09785 at 2223.468 kb on - strand, within BPHYT_RS09785 at 2223.836 kb on + strand at 2223.836 kb on + strand at 2223.837 kb on - strand at 2223.891 kb on - strand at 2223.985 kb on - strand at 2224.091 kb on + strand at 2224.091 kb on + strand at 2224.091 kb on + strand at 2224.092 kb on - strand at 2224.116 kb on + strand at 2224.117 kb on - strand at 2224.205 kb on + strand, within BPHYT_RS09790 at 2224.295 kb on + strand, within BPHYT_RS09790 at 2224.340 kb on + strand, within BPHYT_RS09790 at 2224.520 kb on + strand, within BPHYT_RS09790 at 2224.520 kb on + strand, within BPHYT_RS09790 at 2224.521 kb on - strand, within BPHYT_RS09790 at 2224.859 kb on + strand, within BPHYT_RS09790
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO11 MOI 1 remove 2,220,438 + BPHYT_RS09780 0.32 +0.6 2,220,750 + BPHYT_RS09780 0.53 -0.4 2,221,599 - -0.6 2,221,764 - BPHYT_RS09785 0.13 -0.9 2,221,764 - BPHYT_RS09785 0.13 +0.4 2,221,811 + BPHYT_RS09785 0.15 -0.2 2,221,886 + BPHYT_RS09785 0.18 -1.4 2,221,887 - BPHYT_RS09785 0.18 -0.1 2,221,932 - BPHYT_RS09785 0.20 -1.2 2,222,084 + BPHYT_RS09785 0.26 -0.5 2,222,084 + BPHYT_RS09785 0.26 -0.8 2,222,191 + BPHYT_RS09785 0.31 +0.4 2,222,693 + BPHYT_RS09785 0.51 -0.2 2,222,704 + BPHYT_RS09785 0.52 +0.9 2,223,467 + BPHYT_RS09785 0.83 -1.7 2,223,468 - BPHYT_RS09785 0.83 -0.4 2,223,836 + +2.2 2,223,836 + -0.7 2,223,837 - -0.5 2,223,891 - +2.5 2,223,985 - +2.2 2,224,091 + -1.9 2,224,091 + -0.3 2,224,091 + -0.6 2,224,092 - -0.4 2,224,116 + +0.6 2,224,117 - +1.2 2,224,205 + BPHYT_RS09790 0.16 -1.5 2,224,295 + BPHYT_RS09790 0.24 -0.7 2,224,340 + BPHYT_RS09790 0.27 -0.1 2,224,520 + BPHYT_RS09790 0.42 -2.4 2,224,520 + BPHYT_RS09790 0.42 -1.1 2,224,521 - BPHYT_RS09790 0.42 -1.1 2,224,859 + BPHYT_RS09790 0.70 +0.3
Or see this region's nucleotide sequence