Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS08190

Experiment: 24hr PHYTO11 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS08185 and BPHYT_RS08190 overlap by 8 nucleotidesBPHYT_RS08190 and BPHYT_RS08195 are separated by 2 nucleotides BPHYT_RS08185: BPHYT_RS08185 - pilus assembly protein CpaF, at 1,871,531 to 1,872,940 _RS08185 BPHYT_RS08190: BPHYT_RS08190 - type II secretion system protein, at 1,872,933 to 1,873,961 _RS08190 BPHYT_RS08195: BPHYT_RS08195 - type II secretion system protein, at 1,873,964 to 1,874,974 _RS08195 Position (kb) 1872 1873 1874Strain fitness (log2 ratio) -1 0 1 2at 1872.128 kb on - strand, within BPHYT_RS08185at 1872.382 kb on + strand, within BPHYT_RS08185at 1872.612 kb on + strand, within BPHYT_RS08185at 1873.845 kb on - strand, within BPHYT_RS08190at 1873.894 kb on - strandat 1873.927 kb on - strandat 1874.321 kb on - strand, within BPHYT_RS08195

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Per-strain Table

Position Strand Gene LocusTag Fraction 24hr PHYTO11 MOI 1
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1,872,128 - BPHYT_RS08185 0.42 +0.8
1,872,382 + BPHYT_RS08185 0.60 -1.7
1,872,612 + BPHYT_RS08185 0.77 -0.6
1,873,845 - BPHYT_RS08190 0.89 +0.2
1,873,894 - +0.2
1,873,927 - +0.2
1,874,321 - BPHYT_RS08195 0.35 +1.8

Or see this region's nucleotide sequence