Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS08175

Experiment: 24hr PHYTO11 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS08170 and BPHYT_RS08175 are separated by 42 nucleotidesBPHYT_RS08175 and BPHYT_RS08180 are separated by 132 nucleotides BPHYT_RS08170: BPHYT_RS08170 - pilus assembly protein CpaB, at 1,867,754 to 1,868,704 _RS08170 BPHYT_RS08175: BPHYT_RS08175 - membrane protein, at 1,868,747 to 1,870,153 _RS08175 BPHYT_RS08180: BPHYT_RS08180 - fimbrial protein, at 1,870,286 to 1,871,527 _RS08180 Position (kb) 1868 1869 1870 1871Strain fitness (log2 ratio) -2 -1 0 1 2at 1868.606 kb on - strand, within BPHYT_RS08170at 1868.647 kb on + strandat 1868.648 kb on - strandat 1868.648 kb on - strandat 1868.648 kb on - strandat 1868.744 kb on + strandat 1869.102 kb on - strand, within BPHYT_RS08175at 1869.312 kb on + strand, within BPHYT_RS08175at 1870.190 kb on + strandat 1870.190 kb on + strandat 1870.190 kb on + strandat 1870.527 kb on + strand, within BPHYT_RS08180at 1870.527 kb on + strand, within BPHYT_RS08180at 1870.527 kb on + strand, within BPHYT_RS08180at 1870.528 kb on - strand, within BPHYT_RS08180at 1871.019 kb on - strand, within BPHYT_RS08180

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Per-strain Table

Position Strand Gene LocusTag Fraction 24hr PHYTO11 MOI 1
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1,868,606 - BPHYT_RS08170 0.90 -1.3
1,868,647 + -1.5
1,868,648 - -0.4
1,868,648 - -1.9
1,868,648 - +2.2
1,868,744 + -0.7
1,869,102 - BPHYT_RS08175 0.25 +0.4
1,869,312 + BPHYT_RS08175 0.40 +1.6
1,870,190 + +0.5
1,870,190 + -1.0
1,870,190 + -0.4
1,870,527 + BPHYT_RS08180 0.19 -0.7
1,870,527 + BPHYT_RS08180 0.19 -1.4
1,870,527 + BPHYT_RS08180 0.19 -1.2
1,870,528 - BPHYT_RS08180 0.19 -2.2
1,871,019 - BPHYT_RS08180 0.59 +0.3

Or see this region's nucleotide sequence