Experiment: 24hr PHYTO9 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS28855 and BPHYT_RS28860 are separated by 137 nucleotides BPHYT_RS28860 and BPHYT_RS28865 are separated by 20 nucleotides BPHYT_RS28865 and BPHYT_RS28870 are separated by 73 nucleotides BPHYT_RS28870 and BPHYT_RS28875 are separated by 180 nucleotides
BPHYT_RS28855: BPHYT_RS28855 - LysR family transcriptional regulator, at 2,024,430 to 2,025,335
_RS28855
BPHYT_RS28860: BPHYT_RS28860 - haloacid dehalogenase, at 2,025,473 to 2,026,144
_RS28860
BPHYT_RS28865: BPHYT_RS28865 - alanine racemase, at 2,026,165 to 2,027,304
_RS28865
BPHYT_RS28870: BPHYT_RS28870 - MarR family transcriptional regulator, at 2,027,378 to 2,027,785
_RS28870
BPHYT_RS28875: BPHYT_RS28875 - 5-oxo-1,2,5-tricarboxylic-3-penten acid decarboxylase, at 2,027,966 to 2,028,739
_RS28875
Position (kb)
2026
2027
2028 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2025.406 kb on - strand at 2025.436 kb on - strand at 2026.017 kb on - strand, within BPHYT_RS28860 at 2027.094 kb on - strand, within BPHYT_RS28865 at 2027.511 kb on - strand, within BPHYT_RS28870 at 2027.865 kb on + strand at 2027.911 kb on + strand at 2028.052 kb on - strand, within BPHYT_RS28875 at 2028.096 kb on - strand, within BPHYT_RS28875
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO9 MOI 0.1 remove 2,025,406 - +2.0 2,025,436 - -0.3 2,026,017 - BPHYT_RS28860 0.81 -1.8 2,027,094 - BPHYT_RS28865 0.81 +2.2 2,027,511 - BPHYT_RS28870 0.33 -0.8 2,027,865 + -0.0 2,027,911 + -0.3 2,028,052 - BPHYT_RS28875 0.11 -1.0 2,028,096 - BPHYT_RS28875 0.17 -0.3
Or see this region's nucleotide sequence