Experiment: 24hr PHYTO9 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS27050 and BPHYT_RS27055 are separated by 90 nucleotides BPHYT_RS27055 and BPHYT_RS27060 are separated by 347 nucleotides BPHYT_RS27060 and BPHYT_RS27065 are separated by 162 nucleotides BPHYT_RS27065 and BPHYT_RS27070 are separated by 91 nucleotides
BPHYT_RS27050: BPHYT_RS27050 - DNA topoisomerase, at 1,643,363 to 1,644,682
_RS27050
BPHYT_RS27055: BPHYT_RS27055 - hypothetical protein, at 1,644,773 to 1,645,066
_RS27055
BPHYT_RS27060: BPHYT_RS27060 - photosystem reaction center subunit H, at 1,645,414 to 1,645,911
_RS27060
BPHYT_RS27065: BPHYT_RS27065 - hypothetical protein, at 1,646,074 to 1,646,463
_RS27065
BPHYT_RS27070: BPHYT_RS27070 - cation transporter, at 1,646,555 to 1,647,874
_RS27070
Position (kb) 1645
1646 Strain fitness (log2 ratio) -2
-1
0
1 at 1644.787 kb on + strand at 1645.082 kb on + strand at 1645.142 kb on + strand at 1645.142 kb on + strand at 1645.792 kb on + strand, within BPHYT_RS27060 at 1645.795 kb on + strand, within BPHYT_RS27060 at 1645.796 kb on - strand, within BPHYT_RS27060 at 1646.367 kb on + strand, within BPHYT_RS27065 at 1646.603 kb on - strand at 1646.740 kb on + strand, within BPHYT_RS27070 at 1646.741 kb on - strand, within BPHYT_RS27070
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO9 MOI 0.1 remove 1,644,787 + -0.3 1,645,082 + -0.5 1,645,142 + -1.2 1,645,142 + -2.4 1,645,792 + BPHYT_RS27060 0.76 -2.0 1,645,795 + BPHYT_RS27060 0.77 -1.5 1,645,796 - BPHYT_RS27060 0.77 -1.3 1,646,367 + BPHYT_RS27065 0.75 +0.2 1,646,603 - -0.5 1,646,740 + BPHYT_RS27070 0.14 +0.6 1,646,741 - BPHYT_RS27070 0.14 -0.4
Or see this region's nucleotide sequence