Experiment: 24hr PHYTO9 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS22360 and BPHYT_RS22365 are separated by 24 nucleotides BPHYT_RS22365 and BPHYT_RS22370 are separated by 75 nucleotides
BPHYT_RS22360: BPHYT_RS22360 - response regulator receiver, at 572,982 to 573,944
_RS22360
BPHYT_RS22365: BPHYT_RS22365 - membrane protein, at 573,969 to 575,411
_RS22365
BPHYT_RS22370: BPHYT_RS22370 - alpha/beta hydrolase, at 575,487 to 576,452
_RS22370
Position (kb) 573
574
575
576 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 572.982 kb on - strand at 573.052 kb on - strand at 573.541 kb on - strand, within BPHYT_RS22360 at 573.541 kb on - strand, within BPHYT_RS22360 at 573.861 kb on + strand at 573.892 kb on + strand at 573.893 kb on - strand at 573.973 kb on - strand at 573.973 kb on - strand at 574.023 kb on + strand at 574.028 kb on - strand at 574.028 kb on - strand at 574.168 kb on - strand, within BPHYT_RS22365 at 574.200 kb on + strand, within BPHYT_RS22365 at 574.260 kb on + strand, within BPHYT_RS22365 at 574.286 kb on - strand, within BPHYT_RS22365 at 574.512 kb on + strand, within BPHYT_RS22365 at 574.524 kb on + strand, within BPHYT_RS22365 at 574.525 kb on - strand, within BPHYT_RS22365 at 574.753 kb on - strand, within BPHYT_RS22365 at 575.097 kb on - strand, within BPHYT_RS22365 at 575.181 kb on + strand, within BPHYT_RS22365 at 575.652 kb on - strand, within BPHYT_RS22370 at 575.652 kb on - strand, within BPHYT_RS22370 at 575.817 kb on + strand, within BPHYT_RS22370 at 575.907 kb on + strand, within BPHYT_RS22370 at 575.916 kb on + strand, within BPHYT_RS22370 at 575.916 kb on + strand, within BPHYT_RS22370 at 576.347 kb on - strand, within BPHYT_RS22370
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO9 MOI 0.1 remove 572,982 - +2.6 573,052 - +0.5 573,541 - BPHYT_RS22360 0.58 +1.2 573,541 - BPHYT_RS22360 0.58 +2.1 573,861 + -0.2 573,892 + +0.6 573,893 - -0.4 573,973 - -1.2 573,973 - -0.1 574,023 + +0.2 574,028 - -1.6 574,028 - +0.1 574,168 - BPHYT_RS22365 0.14 -2.8 574,200 + BPHYT_RS22365 0.16 +1.1 574,260 + BPHYT_RS22365 0.20 -1.1 574,286 - BPHYT_RS22365 0.22 +2.4 574,512 + BPHYT_RS22365 0.38 +0.8 574,524 + BPHYT_RS22365 0.38 -0.5 574,525 - BPHYT_RS22365 0.39 +0.3 574,753 - BPHYT_RS22365 0.54 +0.4 575,097 - BPHYT_RS22365 0.78 -0.8 575,181 + BPHYT_RS22365 0.84 -0.1 575,652 - BPHYT_RS22370 0.17 +0.2 575,652 - BPHYT_RS22370 0.17 -0.6 575,817 + BPHYT_RS22370 0.34 +0.6 575,907 + BPHYT_RS22370 0.43 -0.1 575,916 + BPHYT_RS22370 0.44 -1.3 575,916 + BPHYT_RS22370 0.44 +0.2 576,347 - BPHYT_RS22370 0.89 +0.5
Or see this region's nucleotide sequence