Experiment: 24hr PHYTO9 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS20445 and BPHYT_RS20450 are separated by 51 nucleotides BPHYT_RS20450 and BPHYT_RS20455 are separated by 108 nucleotides
BPHYT_RS20445: BPHYT_RS20445 - NAD-dependent dehydratase, at 149,568 to 150,632
_RS20445
BPHYT_RS20450: BPHYT_RS20450 - 6-chlorohydroxyquinol-1,2-dioxygenase, at 150,684 to 151,547
_RS20450
BPHYT_RS20455: BPHYT_RS20455 - hypothetical protein, at 151,656 to 152,333
_RS20455
Position (kb) 150
151
152 Strain fitness (log2 ratio) -1
0
1 at 150.067 kb on + strand, within BPHYT_RS20445 at 150.068 kb on - strand, within BPHYT_RS20445 at 150.196 kb on + strand, within BPHYT_RS20445 at 150.196 kb on + strand, within BPHYT_RS20445 at 150.197 kb on - strand, within BPHYT_RS20445 at 150.199 kb on + strand, within BPHYT_RS20445 at 150.199 kb on + strand, within BPHYT_RS20445 at 150.290 kb on - strand, within BPHYT_RS20445 at 150.290 kb on - strand, within BPHYT_RS20445 at 150.638 kb on + strand at 150.638 kb on + strand at 150.874 kb on + strand, within BPHYT_RS20450 at 150.944 kb on + strand, within BPHYT_RS20450 at 151.078 kb on + strand, within BPHYT_RS20450 at 151.079 kb on - strand, within BPHYT_RS20450 at 151.079 kb on - strand, within BPHYT_RS20450 at 151.664 kb on + strand at 151.878 kb on - strand, within BPHYT_RS20455 at 152.018 kb on - strand, within BPHYT_RS20455 at 152.018 kb on - strand, within BPHYT_RS20455 at 152.058 kb on - strand, within BPHYT_RS20455 at 152.142 kb on - strand, within BPHYT_RS20455 at 152.153 kb on + strand, within BPHYT_RS20455 at 152.167 kb on + strand, within BPHYT_RS20455 at 152.423 kb on + strand at 152.475 kb on + strand at 152.517 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO9 MOI 0.1 remove 150,067 + BPHYT_RS20445 0.47 +0.2 150,068 - BPHYT_RS20445 0.47 +0.5 150,196 + BPHYT_RS20445 0.59 -0.8 150,196 + BPHYT_RS20445 0.59 +1.4 150,197 - BPHYT_RS20445 0.59 -1.3 150,199 + BPHYT_RS20445 0.59 +0.2 150,199 + BPHYT_RS20445 0.59 +0.7 150,290 - BPHYT_RS20445 0.68 -0.9 150,290 - BPHYT_RS20445 0.68 +0.1 150,638 + -0.9 150,638 + +0.4 150,874 + BPHYT_RS20450 0.22 -0.2 150,944 + BPHYT_RS20450 0.30 -0.7 151,078 + BPHYT_RS20450 0.46 +0.5 151,079 - BPHYT_RS20450 0.46 +0.5 151,079 - BPHYT_RS20450 0.46 +0.4 151,664 + +0.1 151,878 - BPHYT_RS20455 0.33 -0.0 152,018 - BPHYT_RS20455 0.53 +0.3 152,018 - BPHYT_RS20455 0.53 -0.1 152,058 - BPHYT_RS20455 0.59 -0.5 152,142 - BPHYT_RS20455 0.72 +0.3 152,153 + BPHYT_RS20455 0.73 -0.0 152,167 + BPHYT_RS20455 0.75 +0.3 152,423 + +0.0 152,475 + +0.7 152,517 - +0.5
Or see this region's nucleotide sequence