Experiment: 24hr PHYTO9 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS16630 and BPHYT_RS16635 are separated by 104 nucleotides BPHYT_RS16635 and BPHYT_RS16640 are separated by 31 nucleotides BPHYT_RS16640 and BPHYT_RS16645 are separated by 206 nucleotides
BPHYT_RS16630: BPHYT_RS16630 - phosphoglycerate mutase, at 3,767,588 to 3,768,259
_RS16630
BPHYT_RS16635: BPHYT_RS16635 - D-aminoacyl-tRNA deacylase, at 3,768,364 to 3,768,822
_RS16635
BPHYT_RS16640: BPHYT_RS16640 - tyrosyl-tRNA synthetase, at 3,768,854 to 3,770,104
_RS16640
BPHYT_RS16645: BPHYT_RS16645 - anhydro-N-acetylmuramic acid kinase, at 3,770,311 to 3,771,441
_RS16645
Position (kb)
3768
3769
3770
3771 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3767.866 kb on - strand, within BPHYT_RS16630 at 3767.866 kb on - strand, within BPHYT_RS16630 at 3767.866 kb on - strand, within BPHYT_RS16630 at 3767.932 kb on - strand, within BPHYT_RS16630 at 3767.932 kb on - strand, within BPHYT_RS16630 at 3767.932 kb on - strand, within BPHYT_RS16630 at 3767.965 kb on + strand, within BPHYT_RS16630 at 3767.989 kb on - strand, within BPHYT_RS16630 at 3770.145 kb on - strand at 3770.145 kb on - strand at 3770.145 kb on - strand at 3770.243 kb on - strand at 3770.264 kb on + strand at 3770.265 kb on - strand at 3770.272 kb on + strand at 3770.272 kb on + strand at 3770.542 kb on + strand, within BPHYT_RS16645 at 3770.650 kb on + strand, within BPHYT_RS16645 at 3770.651 kb on - strand, within BPHYT_RS16645 at 3770.651 kb on - strand, within BPHYT_RS16645 at 3770.651 kb on - strand, within BPHYT_RS16645 at 3770.653 kb on - strand, within BPHYT_RS16645 at 3770.698 kb on - strand, within BPHYT_RS16645 at 3770.839 kb on - strand, within BPHYT_RS16645
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO9 MOI 0.1 remove 3,767,866 - BPHYT_RS16630 0.41 +2.2 3,767,866 - BPHYT_RS16630 0.41 +3.5 3,767,866 - BPHYT_RS16630 0.41 +0.1 3,767,932 - BPHYT_RS16630 0.51 +0.2 3,767,932 - BPHYT_RS16630 0.51 -0.1 3,767,932 - BPHYT_RS16630 0.51 -0.2 3,767,965 + BPHYT_RS16630 0.56 -0.0 3,767,989 - BPHYT_RS16630 0.60 +0.2 3,770,145 - +2.3 3,770,145 - -0.8 3,770,145 - -0.5 3,770,243 - -0.1 3,770,264 + -0.5 3,770,265 - +0.3 3,770,272 + +0.2 3,770,272 + +0.4 3,770,542 + BPHYT_RS16645 0.20 -1.5 3,770,650 + BPHYT_RS16645 0.30 -0.6 3,770,651 - BPHYT_RS16645 0.30 +0.0 3,770,651 - BPHYT_RS16645 0.30 +0.0 3,770,651 - BPHYT_RS16645 0.30 +0.7 3,770,653 - BPHYT_RS16645 0.30 +0.3 3,770,698 - BPHYT_RS16645 0.34 -2.2 3,770,839 - BPHYT_RS16645 0.47 +1.5
Or see this region's nucleotide sequence