Experiment: 24hr PHYTO9 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS14995 and BPHYT_RS15000 are separated by 8 nucleotides BPHYT_RS15000 and BPHYT_RS15005 are separated by 204 nucleotides BPHYT_RS15005 and BPHYT_RS15010 are separated by 147 nucleotides
BPHYT_RS14995: BPHYT_RS14995 - formate dehydrogenase subunit alpha, at 3,408,131 to 3,411,079
_RS14995
BPHYT_RS15000: BPHYT_RS15000 - formate dehydrogenase subunit delta, at 3,411,088 to 3,411,339
_RS15000
BPHYT_RS15005: BPHYT_RS15005 - FAD-binding monooxygenase, at 3,411,544 to 3,413,268
_RS15005
BPHYT_RS15010: BPHYT_RS15010 - cation transporter, at 3,413,416 to 3,414,516
_RS15010
Position (kb)
3411
3412
3413
3414 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3410.675 kb on + strand, within BPHYT_RS14995 at 3410.914 kb on + strand at 3410.967 kb on + strand at 3410.968 kb on - strand at 3410.968 kb on - strand at 3411.338 kb on - strand at 3411.650 kb on + strand at 3411.651 kb on - strand at 3411.651 kb on - strand at 3411.671 kb on + strand at 3411.671 kb on + strand at 3411.672 kb on - strand at 3411.672 kb on - strand at 3411.672 kb on - strand at 3411.696 kb on - strand at 3411.696 kb on - strand at 3412.226 kb on + strand, within BPHYT_RS15005 at 3412.654 kb on - strand, within BPHYT_RS15005 at 3412.791 kb on - strand, within BPHYT_RS15005 at 3413.297 kb on + strand at 3413.297 kb on + strand at 3413.298 kb on - strand at 3413.302 kb on + strand at 3413.303 kb on - strand at 3413.515 kb on + strand at 3413.803 kb on - strand, within BPHYT_RS15010 at 3413.803 kb on - strand, within BPHYT_RS15010 at 3413.804 kb on + strand, within BPHYT_RS15010 at 3413.862 kb on + strand, within BPHYT_RS15010 at 3413.863 kb on - strand, within BPHYT_RS15010 at 3414.100 kb on - strand, within BPHYT_RS15010
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO9 MOI 0.1 remove 3,410,675 + BPHYT_RS14995 0.86 -0.6 3,410,914 + -1.6 3,410,967 + -0.1 3,410,968 - +0.2 3,410,968 - +1.5 3,411,338 - +0.7 3,411,650 + -0.5 3,411,651 - -0.4 3,411,651 - -0.3 3,411,671 + -1.1 3,411,671 + +2.8 3,411,672 - -1.1 3,411,672 - -1.2 3,411,672 - +0.6 3,411,696 - +0.2 3,411,696 - -1.5 3,412,226 + BPHYT_RS15005 0.40 +1.5 3,412,654 - BPHYT_RS15005 0.64 -0.6 3,412,791 - BPHYT_RS15005 0.72 +1.0 3,413,297 + -1.3 3,413,297 + -1.2 3,413,298 - -3.0 3,413,302 + -1.8 3,413,303 - +0.9 3,413,515 + +0.4 3,413,803 - BPHYT_RS15010 0.35 +0.5 3,413,803 - BPHYT_RS15010 0.35 +0.7 3,413,804 + BPHYT_RS15010 0.35 -1.6 3,413,862 + BPHYT_RS15010 0.41 -0.3 3,413,863 - BPHYT_RS15010 0.41 -0.1 3,414,100 - BPHYT_RS15010 0.62 +0.7
Or see this region's nucleotide sequence