Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS10885

Experiment: 24hr PHYTO9 MOI 0.1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS10875 and BPHYT_RS10880 are separated by 169 nucleotidesBPHYT_RS10880 and BPHYT_RS10885 overlap by 11 nucleotidesBPHYT_RS10885 and BPHYT_RS10890 are separated by 12 nucleotidesBPHYT_RS10890 and BPHYT_RS10895 are separated by 179 nucleotides BPHYT_RS10875: BPHYT_RS10875 - transporter, at 2,473,874 to 2,476,924 _RS10875 BPHYT_RS10880: BPHYT_RS10880 - hopanoid biosynthesis associated protein HpnK, at 2,477,094 to 2,477,918 _RS10880 BPHYT_RS10885: BPHYT_RS10885 - glycosyl transferase, at 2,477,908 to 2,479,215 _RS10885 BPHYT_RS10890: BPHYT_RS10890 - hypothetical protein, at 2,479,228 to 2,479,578 _RS10890 BPHYT_RS10895: BPHYT_RS10895 - transcriptional regulator, at 2,479,758 to 2,480,417 _RS10895 Position (kb) 2477 2478 2479 2480Strain fitness (log2 ratio) -1 0 1 2 3at 2477.095 kb on + strandat 2478.079 kb on - strand, within BPHYT_RS10885at 2478.331 kb on - strand, within BPHYT_RS10885at 2478.971 kb on + strand, within BPHYT_RS10885at 2479.080 kb on - strand, within BPHYT_RS10885at 2479.645 kb on - strandat 2479.709 kb on - strandat 2479.757 kb on + strandat 2479.758 kb on - strandat 2479.758 kb on - strandat 2479.765 kb on + strandat 2479.765 kb on + strandat 2480.050 kb on - strand, within BPHYT_RS10895

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Per-strain Table

Position Strand Gene LocusTag Fraction 24hr PHYTO9 MOI 0.1
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2,477,095 + +0.9
2,478,079 - BPHYT_RS10885 0.13 +0.3
2,478,331 - BPHYT_RS10885 0.32 -0.5
2,478,971 + BPHYT_RS10885 0.81 +0.3
2,479,080 - BPHYT_RS10885 0.90 +1.1
2,479,645 - +2.5
2,479,709 - -0.5
2,479,757 + -1.1
2,479,758 - -0.7
2,479,758 - +3.4
2,479,765 + -0.3
2,479,765 + +0.9
2,480,050 - BPHYT_RS10895 0.44 -1.0

Or see this region's nucleotide sequence