Experiment: 24hr PHYTO9 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS00700 and BPHYT_RS00705 overlap by 1 nucleotides BPHYT_RS00705 and BPHYT_RS00710 overlap by 14 nucleotides BPHYT_RS00710 and BPHYT_RS00715 are separated by 18 nucleotides
BPHYT_RS00700: BPHYT_RS00700 - branched-chain amino acid ABC transporter permease, at 163,772 to 164,854
_RS00700
BPHYT_RS00705: BPHYT_RS00705 - branched-chain amino acid ABC transporter substrate-binding protein, at 164,854 to 165,588
_RS00705
BPHYT_RS00710: BPHYT_RS00710 - branched-chain amino acid ABC transporter ATPase, at 165,575 to 166,318
_RS00710
BPHYT_RS00715: BPHYT_RS00715 - branched-chain amino acid ABC transporter substrate-binding protein, at 166,337 to 167,515
_RS00715
Position (kb) 164
165
166 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 163.962 kb on - strand, within BPHYT_RS00700 at 163.962 kb on - strand, within BPHYT_RS00700 at 164.130 kb on - strand, within BPHYT_RS00700 at 164.340 kb on + strand, within BPHYT_RS00700 at 164.340 kb on + strand, within BPHYT_RS00700 at 164.387 kb on + strand, within BPHYT_RS00700 at 164.589 kb on + strand, within BPHYT_RS00700 at 164.589 kb on + strand, within BPHYT_RS00700 at 164.590 kb on - strand, within BPHYT_RS00700 at 164.672 kb on + strand, within BPHYT_RS00700 at 164.745 kb on + strand, within BPHYT_RS00700 at 165.040 kb on + strand, within BPHYT_RS00705 at 165.040 kb on + strand, within BPHYT_RS00705 at 165.041 kb on - strand, within BPHYT_RS00705 at 165.515 kb on - strand at 165.553 kb on + strand at 165.553 kb on + strand at 165.554 kb on - strand at 165.912 kb on + strand, within BPHYT_RS00710 at 165.912 kb on + strand, within BPHYT_RS00710 at 165.920 kb on + strand, within BPHYT_RS00710 at 166.262 kb on + strand at 166.262 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO9 MOI 0.1 remove 163,962 - BPHYT_RS00700 0.18 +0.5 163,962 - BPHYT_RS00700 0.18 -0.2 164,130 - BPHYT_RS00700 0.33 +0.8 164,340 + BPHYT_RS00700 0.52 +0.3 164,340 + BPHYT_RS00700 0.52 +0.3 164,387 + BPHYT_RS00700 0.57 +0.5 164,589 + BPHYT_RS00700 0.75 +0.0 164,589 + BPHYT_RS00700 0.75 -0.2 164,590 - BPHYT_RS00700 0.76 +0.1 164,672 + BPHYT_RS00700 0.83 -1.8 164,745 + BPHYT_RS00700 0.90 +0.1 165,040 + BPHYT_RS00705 0.25 +0.2 165,040 + BPHYT_RS00705 0.25 -1.2 165,041 - BPHYT_RS00705 0.25 +3.7 165,515 - -0.1 165,553 + +0.4 165,553 + +0.2 165,554 - +0.5 165,912 + BPHYT_RS00710 0.45 -0.2 165,912 + BPHYT_RS00710 0.45 +0.1 165,920 + BPHYT_RS00710 0.46 +0.7 166,262 + -1.7 166,262 + +0.6
Or see this region's nucleotide sequence