Experiment: 24hr PHYTO9 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS36450 and BPHYT_RS36455 are separated by 0 nucleotides BPHYT_RS36455 and BPHYT_RS36460 are separated by 12 nucleotides BPHYT_RS36460 and BPHYT_RS36465 are separated by 58 nucleotides BPHYT_RS36465 and BPHYT_RS36470 are separated by 252 nucleotides
BPHYT_RS36450: BPHYT_RS36450 - hypothetical protein, at 101,360 to 102,559
_RS36450
BPHYT_RS36455: BPHYT_RS36455 - hypothetical protein, at 102,560 to 102,778
_RS36455
BPHYT_RS36460: BPHYT_RS36460 - hypothetical protein, at 102,791 to 103,294
_RS36460
BPHYT_RS36465: BPHYT_RS36465 - DNA-binding protein, at 103,353 to 103,673
_RS36465
BPHYT_RS36470: BPHYT_RS36470 - hypothetical protein, at 103,926 to 104,846
_RS36470
Position (kb) 103
104 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 102.558 kb on - strand at 102.741 kb on - strand, within BPHYT_RS36455 at 102.816 kb on - strand at 102.841 kb on - strand at 103.020 kb on - strand, within BPHYT_RS36460 at 103.023 kb on - strand, within BPHYT_RS36460 at 103.261 kb on - strand at 103.261 kb on - strand at 103.293 kb on - strand at 103.322 kb on - strand at 103.482 kb on + strand, within BPHYT_RS36465 at 103.749 kb on + strand at 103.749 kb on + strand at 103.750 kb on - strand at 103.939 kb on + strand at 103.939 kb on - strand at 103.939 kb on - strand at 103.989 kb on + strand at 104.014 kb on - strand at 104.081 kb on + strand, within BPHYT_RS36470 at 104.082 kb on - strand, within BPHYT_RS36470
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO9 MOI 10 remove 102,558 - -0.1 102,741 - BPHYT_RS36455 0.83 -0.7 102,816 - -0.1 102,841 - +1.1 103,020 - BPHYT_RS36460 0.45 -2.8 103,023 - BPHYT_RS36460 0.46 -0.9 103,261 - +0.2 103,261 - -0.2 103,293 - +0.3 103,322 - -0.2 103,482 + BPHYT_RS36465 0.40 +2.2 103,749 + -0.2 103,749 + -0.8 103,750 - +1.5 103,939 + +2.2 103,939 - +0.4 103,939 - -1.2 103,989 + -0.3 104,014 - +0.2 104,081 + BPHYT_RS36470 0.17 -1.0 104,082 - BPHYT_RS36470 0.17 -1.7
Or see this region's nucleotide sequence