Experiment: 24hr PHYTO9 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS26275 and BPHYT_RS26280 are separated by 145 nucleotides BPHYT_RS26280 and BPHYT_RS26285 are separated by 152 nucleotides BPHYT_RS26285 and BPHYT_RS26290 are separated by 265 nucleotides BPHYT_RS26290 and BPHYT_RS26295 are separated by 207 nucleotides
BPHYT_RS26275: BPHYT_RS26275 - membrane protein, at 1,465,069 to 1,467,102
_RS26275
BPHYT_RS26280: BPHYT_RS26280 - hypothetical protein, at 1,467,248 to 1,467,427
_RS26280
BPHYT_RS26285: BPHYT_RS26285 - glycogen debranching protein, at 1,467,580 to 1,469,796
_RS26285
BPHYT_RS26290: BPHYT_RS26290 - photosystem reaction center subunit H, at 1,470,062 to 1,470,469
_RS26290
BPHYT_RS26295: BPHYT_RS26295 - chemotaxis protein CheY, at 1,470,677 to 1,471,135
_RS26295
Position (kb) 1467
1468
1469
1470 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 1467.187 kb on - strand at 1467.187 kb on - strand at 1467.250 kb on - strand at 1467.306 kb on - strand, within BPHYT_RS26280 at 1467.379 kb on - strand, within BPHYT_RS26280 at 1467.474 kb on - strand at 1468.155 kb on - strand, within BPHYT_RS26285 at 1468.383 kb on - strand, within BPHYT_RS26285 at 1468.383 kb on - strand, within BPHYT_RS26285 at 1468.857 kb on - strand, within BPHYT_RS26285 at 1469.123 kb on + strand, within BPHYT_RS26285 at 1469.615 kb on + strand at 1469.796 kb on + strand at 1469.797 kb on - strand at 1470.128 kb on + strand, within BPHYT_RS26290 at 1470.256 kb on + strand, within BPHYT_RS26290 at 1470.312 kb on - strand, within BPHYT_RS26290
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO9 MOI 10 remove 1,467,187 - +0.9 1,467,187 - +0.3 1,467,250 - +0.3 1,467,306 - BPHYT_RS26280 0.32 -2.2 1,467,379 - BPHYT_RS26280 0.73 -2.0 1,467,474 - +0.3 1,468,155 - BPHYT_RS26285 0.26 -1.3 1,468,383 - BPHYT_RS26285 0.36 +3.4 1,468,383 - BPHYT_RS26285 0.36 +3.1 1,468,857 - BPHYT_RS26285 0.58 +0.4 1,469,123 + BPHYT_RS26285 0.70 -1.8 1,469,615 + +0.6 1,469,796 + +0.9 1,469,797 - +0.3 1,470,128 + BPHYT_RS26290 0.16 -3.1 1,470,256 + BPHYT_RS26290 0.48 -0.3 1,470,312 - BPHYT_RS26290 0.61 -0.2
Or see this region's nucleotide sequence