Experiment: 24hr PHYTO9 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS20425 and BPHYT_RS20430 are separated by 252 nucleotides BPHYT_RS20430 and BPHYT_RS20435 are separated by 60 nucleotides BPHYT_RS20435 and BPHYT_RS20440 are separated by 238 nucleotides
BPHYT_RS20425: BPHYT_RS20425 - hypothetical protein, at 146,078 to 146,386
_RS20425
BPHYT_RS20430: BPHYT_RS20430 - 3-dehydroquinate dehydratase, at 146,639 to 147,094
_RS20430
BPHYT_RS20435: BPHYT_RS20435 - MFS transporter, at 147,155 to 148,531
_RS20435
BPHYT_RS20440: BPHYT_RS20440 - IclR family transcriptional regulator, at 148,770 to 149,552
_RS20440
Position (kb) 147
148
149 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 146.304 kb on - strand, within BPHYT_RS20425 at 146.307 kb on - strand, within BPHYT_RS20425 at 146.466 kb on + strand at 146.466 kb on + strand at 146.467 kb on - strand at 146.467 kb on - strand at 146.509 kb on - strand at 146.596 kb on + strand at 146.596 kb on + strand at 146.597 kb on - strand at 146.792 kb on - strand, within BPHYT_RS20430 at 146.792 kb on - strand, within BPHYT_RS20430 at 147.074 kb on + strand at 147.075 kb on - strand at 147.075 kb on - strand at 147.078 kb on + strand at 147.079 kb on - strand at 147.079 kb on - strand at 147.079 kb on - strand at 147.079 kb on - strand at 147.080 kb on + strand at 147.080 kb on + strand at 147.153 kb on + strand at 147.156 kb on + strand at 147.190 kb on + strand at 147.402 kb on + strand, within BPHYT_RS20435 at 147.556 kb on - strand, within BPHYT_RS20435 at 147.619 kb on + strand, within BPHYT_RS20435 at 147.753 kb on + strand, within BPHYT_RS20435 at 147.908 kb on - strand, within BPHYT_RS20435 at 147.909 kb on + strand, within BPHYT_RS20435 at 147.910 kb on - strand, within BPHYT_RS20435 at 147.910 kb on - strand, within BPHYT_RS20435 at 147.910 kb on - strand, within BPHYT_RS20435 at 147.964 kb on - strand, within BPHYT_RS20435 at 148.065 kb on + strand, within BPHYT_RS20435 at 148.065 kb on + strand, within BPHYT_RS20435 at 148.066 kb on - strand, within BPHYT_RS20435 at 148.185 kb on - strand, within BPHYT_RS20435 at 148.675 kb on - strand at 149.426 kb on + strand, within BPHYT_RS20440
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO9 MOI 10 remove 146,304 - BPHYT_RS20425 0.73 -0.9 146,307 - BPHYT_RS20425 0.74 +1.6 146,466 + -1.0 146,466 + +1.2 146,467 - -1.4 146,467 - -1.3 146,509 - +0.2 146,596 + -0.5 146,596 + +0.9 146,597 - -0.6 146,792 - BPHYT_RS20430 0.34 -0.0 146,792 - BPHYT_RS20430 0.34 -0.4 147,074 + -2.2 147,075 - -0.8 147,075 - +0.5 147,078 + -2.9 147,079 - +0.4 147,079 - -0.6 147,079 - +0.4 147,079 - +1.0 147,080 + +0.4 147,080 + -0.1 147,153 + -0.6 147,156 + +0.2 147,190 + +0.1 147,402 + BPHYT_RS20435 0.18 -0.1 147,556 - BPHYT_RS20435 0.29 -2.3 147,619 + BPHYT_RS20435 0.34 -0.9 147,753 + BPHYT_RS20435 0.43 -0.4 147,908 - BPHYT_RS20435 0.55 -1.7 147,909 + BPHYT_RS20435 0.55 -0.3 147,910 - BPHYT_RS20435 0.55 -1.8 147,910 - BPHYT_RS20435 0.55 +1.1 147,910 - BPHYT_RS20435 0.55 -1.3 147,964 - BPHYT_RS20435 0.59 +0.6 148,065 + BPHYT_RS20435 0.66 +0.2 148,065 + BPHYT_RS20435 0.66 -1.5 148,066 - BPHYT_RS20435 0.66 +0.9 148,185 - BPHYT_RS20435 0.75 +0.4 148,675 - +0.2 149,426 + BPHYT_RS20440 0.84 -3.1
Or see this region's nucleotide sequence