Experiment: 24hr PHYTO9 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS13705 and BPHYT_RS13710 are separated by 373 nucleotides BPHYT_RS13710 and BPHYT_RS13715 are separated by 279 nucleotides BPHYT_RS13715 and BPHYT_RS13720 are separated by 240 nucleotides
BPHYT_RS13705: BPHYT_RS13705 - chorismate synthase, at 3,122,861 to 3,123,961
_RS13705
BPHYT_RS13710: BPHYT_RS13710 - glycerol acyltransferase, at 3,124,335 to 3,126,272
_RS13710
BPHYT_RS13715: BPHYT_RS13715 - hypothetical protein, at 3,126,552 to 3,127,013
_RS13715
BPHYT_RS13720: BPHYT_RS13720 - O-acetylhomoserine aminocarboxypropyltransferase, at 3,127,254 to 3,128,606
_RS13720
Position (kb) 3124
3125
3126
3127 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 3123.495 kb on + strand, within BPHYT_RS13705 at 3123.495 kb on + strand, within BPHYT_RS13705 at 3123.496 kb on - strand, within BPHYT_RS13705 at 3123.630 kb on + strand, within BPHYT_RS13705 at 3123.630 kb on + strand, within BPHYT_RS13705 at 3123.995 kb on - strand at 3123.995 kb on - strand at 3123.997 kb on + strand at 3123.998 kb on - strand at 3123.998 kb on - strand at 3123.998 kb on - strand at 3124.062 kb on - strand at 3124.062 kb on - strand at 3124.063 kb on + strand at 3124.063 kb on + strand at 3124.064 kb on - strand at 3124.212 kb on + strand at 3124.212 kb on + strand at 3124.707 kb on - strand, within BPHYT_RS13710 at 3124.961 kb on - strand, within BPHYT_RS13710 at 3124.966 kb on + strand, within BPHYT_RS13710 at 3124.966 kb on + strand, within BPHYT_RS13710 at 3124.966 kb on + strand, within BPHYT_RS13710 at 3125.149 kb on + strand, within BPHYT_RS13710 at 3125.149 kb on + strand, within BPHYT_RS13710 at 3125.149 kb on + strand, within BPHYT_RS13710 at 3125.304 kb on + strand, within BPHYT_RS13710 at 3125.446 kb on - strand at 3125.538 kb on - strand, within BPHYT_RS13710 at 3125.824 kb on + strand, within BPHYT_RS13710 at 3125.825 kb on - strand, within BPHYT_RS13710 at 3125.825 kb on - strand, within BPHYT_RS13710 at 3125.825 kb on - strand, within BPHYT_RS13710 at 3125.830 kb on + strand, within BPHYT_RS13710 at 3125.831 kb on - strand, within BPHYT_RS13710 at 3125.884 kb on + strand, within BPHYT_RS13710 at 3126.101 kb on - strand at 3126.599 kb on + strand, within BPHYT_RS13715 at 3126.600 kb on - strand, within BPHYT_RS13715 at 3126.601 kb on + strand, within BPHYT_RS13715 at 3126.601 kb on + strand, within BPHYT_RS13715 at 3126.601 kb on + strand, within BPHYT_RS13715 at 3126.601 kb on + strand, within BPHYT_RS13715 at 3126.602 kb on - strand, within BPHYT_RS13715 at 3126.602 kb on - strand, within BPHYT_RS13715 at 3126.661 kb on + strand, within BPHYT_RS13715 at 3126.661 kb on + strand, within BPHYT_RS13715 at 3126.886 kb on + strand, within BPHYT_RS13715 at 3126.894 kb on + strand, within BPHYT_RS13715 at 3126.993 kb on - strand at 3127.046 kb on - strand at 3127.084 kb on + strand at 3127.085 kb on - strand at 3127.085 kb on - strand at 3127.199 kb on - strand at 3127.199 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO9 MOI 10 remove 3,123,495 + BPHYT_RS13705 0.58 +1.1 3,123,495 + BPHYT_RS13705 0.58 +0.4 3,123,496 - BPHYT_RS13705 0.58 -3.0 3,123,630 + BPHYT_RS13705 0.70 +0.8 3,123,630 + BPHYT_RS13705 0.70 +2.9 3,123,995 - -0.2 3,123,995 - -0.1 3,123,997 + -0.7 3,123,998 - -0.2 3,123,998 - -0.4 3,123,998 - +0.2 3,124,062 - +0.1 3,124,062 - +0.4 3,124,063 + -1.2 3,124,063 + -0.2 3,124,064 - +2.7 3,124,212 + +0.2 3,124,212 + +0.9 3,124,707 - BPHYT_RS13710 0.19 +1.1 3,124,961 - BPHYT_RS13710 0.32 -1.6 3,124,966 + BPHYT_RS13710 0.33 +0.6 3,124,966 + BPHYT_RS13710 0.33 -1.0 3,124,966 + BPHYT_RS13710 0.33 +0.1 3,125,149 + BPHYT_RS13710 0.42 -1.2 3,125,149 + BPHYT_RS13710 0.42 -0.0 3,125,149 + BPHYT_RS13710 0.42 -1.2 3,125,304 + BPHYT_RS13710 0.50 -0.8 3,125,446 - -1.0 3,125,538 - BPHYT_RS13710 0.62 -1.3 3,125,824 + BPHYT_RS13710 0.77 +0.1 3,125,825 - BPHYT_RS13710 0.77 +0.0 3,125,825 - BPHYT_RS13710 0.77 -1.1 3,125,825 - BPHYT_RS13710 0.77 -0.8 3,125,830 + BPHYT_RS13710 0.77 -0.8 3,125,831 - BPHYT_RS13710 0.77 +0.0 3,125,884 + BPHYT_RS13710 0.80 -0.3 3,126,101 - -1.4 3,126,599 + BPHYT_RS13715 0.10 -1.0 3,126,600 - BPHYT_RS13715 0.10 -0.2 3,126,601 + BPHYT_RS13715 0.11 -1.2 3,126,601 + BPHYT_RS13715 0.11 +0.1 3,126,601 + BPHYT_RS13715 0.11 +0.6 3,126,601 + BPHYT_RS13715 0.11 -0.7 3,126,602 - BPHYT_RS13715 0.11 +1.8 3,126,602 - BPHYT_RS13715 0.11 +0.8 3,126,661 + BPHYT_RS13715 0.24 -0.1 3,126,661 + BPHYT_RS13715 0.24 +1.0 3,126,886 + BPHYT_RS13715 0.72 +0.3 3,126,894 + BPHYT_RS13715 0.74 +1.1 3,126,993 - -1.1 3,127,046 - +0.1 3,127,084 + -0.5 3,127,085 - +0.8 3,127,085 - +0.6 3,127,199 - -1.2 3,127,199 - +0.3
Or see this region's nucleotide sequence