Experiment: 24hr PHYTO9 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS13475 and BPHYT_RS13480 are separated by 170 nucleotides BPHYT_RS13480 and BPHYT_RS13485 are separated by 112 nucleotides BPHYT_RS13485 and BPHYT_RS13490 are separated by 360 nucleotides BPHYT_RS13490 and BPHYT_RS13495 are separated by 30 nucleotides
BPHYT_RS13475: BPHYT_RS13475 - 2-keto-gluconate dehydrogenase, at 3,075,425 to 3,077,011
_RS13475
BPHYT_RS13480: BPHYT_RS13480 - hypothetical protein, at 3,077,182 to 3,077,853
_RS13480
BPHYT_RS13485: BPHYT_RS13485 - lactoylglutathione lyase, at 3,077,966 to 3,078,364
_RS13485
BPHYT_RS13490: BPHYT_RS13490 - hypothetical protein, at 3,078,725 to 3,079,135
_RS13490
BPHYT_RS13495: BPHYT_RS13495 - AsnC family transcriptional regulator, at 3,079,166 to 3,079,690
_RS13495
Position (kb)
3077
3078
3079 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3077.094 kb on + strand at 3077.136 kb on - strand at 3077.183 kb on + strand at 3077.929 kb on - strand at 3077.937 kb on + strand at 3077.964 kb on + strand at 3077.964 kb on + strand at 3078.241 kb on - strand at 3078.654 kb on - strand at 3078.676 kb on + strand at 3078.908 kb on + strand, within BPHYT_RS13490 at 3078.909 kb on - strand, within BPHYT_RS13490 at 3078.981 kb on - strand, within BPHYT_RS13490 at 3079.201 kb on + strand at 3079.201 kb on + strand at 3079.202 kb on - strand at 3079.202 kb on - strand at 3079.215 kb on + strand at 3079.216 kb on - strand at 3079.216 kb on - strand at 3079.353 kb on - strand, within BPHYT_RS13495
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO9 MOI 10 remove 3,077,094 + -1.3 3,077,136 - -0.2 3,077,183 + -0.0 3,077,929 - -1.4 3,077,937 + +2.2 3,077,964 + -0.1 3,077,964 + +1.0 3,078,241 - +0.7 3,078,654 - -0.6 3,078,676 + +0.8 3,078,908 + BPHYT_RS13490 0.45 -1.9 3,078,909 - BPHYT_RS13490 0.45 -0.6 3,078,981 - BPHYT_RS13490 0.62 -0.5 3,079,201 + -0.6 3,079,201 + +1.2 3,079,202 - -0.2 3,079,202 - -0.5 3,079,215 + +0.7 3,079,216 - -0.8 3,079,216 - -0.5 3,079,353 - BPHYT_RS13495 0.36 +0.6
Or see this region's nucleotide sequence