Experiment: 24hr PHYTO9 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS10955 and BPHYT_RS10960 are separated by 592 nucleotides BPHYT_RS10960 and BPHYT_RS10965 are separated by 53 nucleotides BPHYT_RS10965 and BPHYT_RS10970 are separated by 78 nucleotides BPHYT_RS10970 and BPHYT_RS10975 are separated by 57 nucleotides BPHYT_RS10975 and BPHYT_RS10980 are separated by 328 nucleotides
BPHYT_RS10955: BPHYT_RS10955 - dioxygenase, at 2,492,643 to 2,493,575
_RS10955
BPHYT_RS10960: BPHYT_RS10960 - hypothetical protein, at 2,494,168 to 2,494,281
_RS10960
BPHYT_RS10965: BPHYT_RS10965 - hypothetical protein, at 2,494,335 to 2,494,724
_RS10965
BPHYT_RS10970: BPHYT_RS10970 - hypothetical protein, at 2,494,803 to 2,495,051
_RS10970
BPHYT_RS10975: BPHYT_RS10975 - hypothetical protein, at 2,495,109 to 2,495,351
_RS10975
BPHYT_RS10980: BPHYT_RS10980 - 2-dehydro-3-deoxy-6-phosphogalactonate aldolase, at 2,495,680 to 2,496,927
_RS10980
Position (kb)
2494
2495 Strain fitness (log2 ratio)
-2
-1
0
1 at 2493.579 kb on - strand at 2493.579 kb on - strand at 2494.121 kb on - strand at 2494.133 kb on + strand at 2494.317 kb on - strand at 2494.557 kb on + strand, within BPHYT_RS10965 at 2495.160 kb on - strand, within BPHYT_RS10975 at 2495.482 kb on + strand at 2495.483 kb on - strand at 2495.662 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO9 MOI 10 remove 2,493,579 - -0.5 2,493,579 - -0.0 2,494,121 - +0.3 2,494,133 + +0.3 2,494,317 - -1.0 2,494,557 + BPHYT_RS10965 0.57 -0.2 2,495,160 - BPHYT_RS10975 0.21 +0.2 2,495,482 + -2.1 2,495,483 - +0.3 2,495,662 - -0.6
Or see this region's nucleotide sequence