Experiment: 24hr PHYTO9 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS05360 and BPHYT_RS05365 are separated by 155 nucleotides BPHYT_RS05365 and BPHYT_RS05370 are separated by 43 nucleotides BPHYT_RS05370 and BPHYT_RS05375 are separated by 253 nucleotides BPHYT_RS05375 and BPHYT_RS05380 are separated by 152 nucleotides
BPHYT_RS05360: BPHYT_RS05360 - MFS transporter, at 1,233,097 to 1,234,416
_RS05360
BPHYT_RS05365: BPHYT_RS05365 - 3-ketoacyl-ACP reductase, at 1,234,572 to 1,235,330
_RS05365
BPHYT_RS05370: BPHYT_RS05370 - aldehyde dehydrogenase, at 1,235,374 to 1,236,813
_RS05370
BPHYT_RS05375: BPHYT_RS05375 - inorganic pyrophosphatase, at 1,237,067 to 1,237,594
_RS05375
BPHYT_RS05380: BPHYT_RS05380 - transcriptional regulator, at 1,237,747 to 1,238,085
_RS05380
Position (kb)
1235
1236
1237 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1234.448 kb on - strand at 1234.643 kb on - strand at 1234.949 kb on - strand, within BPHYT_RS05365 at 1235.315 kb on + strand at 1235.368 kb on - strand at 1235.417 kb on + strand at 1235.417 kb on + strand at 1235.502 kb on - strand at 1235.502 kb on - strand at 1235.502 kb on - strand at 1236.213 kb on + strand, within BPHYT_RS05370 at 1236.423 kb on - strand, within BPHYT_RS05370 at 1236.423 kb on - strand, within BPHYT_RS05370 at 1236.942 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO9 MOI 10 remove 1,234,448 - -0.7 1,234,643 - -0.5 1,234,949 - BPHYT_RS05365 0.50 +0.3 1,235,315 + -0.5 1,235,368 - +0.2 1,235,417 + -0.8 1,235,417 + -0.5 1,235,502 - +0.5 1,235,502 - +0.5 1,235,502 - -0.2 1,236,213 + BPHYT_RS05370 0.58 -1.7 1,236,423 - BPHYT_RS05370 0.73 -0.3 1,236,423 - BPHYT_RS05370 0.73 -3.2 1,236,942 - -0.0
Or see this region's nucleotide sequence