Experiment: 24hr PHYTO9 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS02060 and BPHYT_RS02065 are separated by 291 nucleotides BPHYT_RS02065 and BPHYT_RS02070 are separated by 562 nucleotides
BPHYT_RS02060: BPHYT_RS02060 - diguanylate cyclase, at 470,506 to 472,356
_RS02060
BPHYT_RS02065: BPHYT_RS02065 - membrane protein, at 472,648 to 473,676
_RS02065
BPHYT_RS02070: BPHYT_RS02070 - hypothetical protein, at 474,239 to 474,784
_RS02070
Position (kb) 472
473
474 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 472.236 kb on - strand at 472.424 kb on - strand at 472.519 kb on - strand at 472.563 kb on + strand at 472.564 kb on - strand at 472.617 kb on + strand at 472.922 kb on - strand, within BPHYT_RS02065 at 473.039 kb on + strand, within BPHYT_RS02065 at 473.178 kb on + strand, within BPHYT_RS02065 at 473.284 kb on + strand, within BPHYT_RS02065 at 473.285 kb on - strand, within BPHYT_RS02065 at 473.344 kb on - strand, within BPHYT_RS02065 at 473.379 kb on - strand, within BPHYT_RS02065 at 473.416 kb on - strand, within BPHYT_RS02065 at 473.837 kb on + strand at 473.838 kb on - strand at 473.838 kb on - strand at 473.838 kb on - strand at 473.843 kb on + strand at 473.844 kb on - strand at 473.844 kb on - strand at 473.844 kb on - strand at 473.946 kb on - strand at 474.039 kb on + strand at 474.040 kb on - strand at 474.107 kb on - strand at 474.107 kb on - strand at 474.442 kb on + strand, within BPHYT_RS02070
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO9 MOI 10 remove 472,236 - +0.5 472,424 - -0.9 472,519 - +0.5 472,563 + -0.4 472,564 - -3.2 472,617 + -0.7 472,922 - BPHYT_RS02065 0.27 +1.4 473,039 + BPHYT_RS02065 0.38 +0.6 473,178 + BPHYT_RS02065 0.52 +2.1 473,284 + BPHYT_RS02065 0.62 +1.6 473,285 - BPHYT_RS02065 0.62 -0.0 473,344 - BPHYT_RS02065 0.68 -0.5 473,379 - BPHYT_RS02065 0.71 -1.1 473,416 - BPHYT_RS02065 0.75 +1.1 473,837 + -0.7 473,838 - -0.3 473,838 - +0.3 473,838 - -2.2 473,843 + -0.8 473,844 - +0.2 473,844 - -1.6 473,844 - -1.5 473,946 - +0.3 474,039 + +0.8 474,040 - -1.1 474,107 - +0.4 474,107 - +0.9 474,442 + BPHYT_RS02070 0.37 -1.2
Or see this region's nucleotide sequence