Experiment: 24hr PHYTO9 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS01875 and BPHYT_RS01880 are separated by 252 nucleotides BPHYT_RS01880 and BPHYT_RS01885 are separated by 423 nucleotides
BPHYT_RS01875: BPHYT_RS01875 - carboxymethylenebutenolidase, at 426,503 to 427,201
_RS01875
BPHYT_RS01880: BPHYT_RS01880 - nitronate monooxygenase, at 427,454 to 428,554
_RS01880
BPHYT_RS01885: BPHYT_RS01885 - branched-chain amino acid ABC transporter substrate-binding protein, at 428,978 to 430,123
_RS01885
Position (kb) 427
428
429 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 426.504 kb on + strand at 426.567 kb on + strand at 426.567 kb on + strand at 426.568 kb on - strand at 426.612 kb on + strand, within BPHYT_RS01875 at 426.613 kb on - strand, within BPHYT_RS01875 at 426.772 kb on - strand, within BPHYT_RS01875 at 426.772 kb on - strand, within BPHYT_RS01875 at 426.813 kb on + strand, within BPHYT_RS01875 at 426.813 kb on + strand, within BPHYT_RS01875 at 426.975 kb on + strand, within BPHYT_RS01875 at 426.975 kb on + strand, within BPHYT_RS01875 at 427.070 kb on - strand, within BPHYT_RS01875 at 427.144 kb on - strand at 427.908 kb on + strand, within BPHYT_RS01880 at 427.909 kb on - strand, within BPHYT_RS01880 at 427.910 kb on + strand, within BPHYT_RS01880 at 427.910 kb on + strand, within BPHYT_RS01880 at 427.910 kb on + strand, within BPHYT_RS01880 at 427.910 kb on + strand, within BPHYT_RS01880 at 427.910 kb on + strand, within BPHYT_RS01880 at 427.914 kb on + strand, within BPHYT_RS01880 at 427.914 kb on + strand, within BPHYT_RS01880 at 427.914 kb on + strand, within BPHYT_RS01880 at 427.914 kb on + strand, within BPHYT_RS01880 at 427.914 kb on + strand, within BPHYT_RS01880 at 427.914 kb on + strand, within BPHYT_RS01880 at 427.914 kb on + strand, within BPHYT_RS01880 at 427.915 kb on - strand, within BPHYT_RS01880 at 427.915 kb on - strand, within BPHYT_RS01880 at 427.915 kb on - strand, within BPHYT_RS01880 at 427.917 kb on + strand, within BPHYT_RS01880 at 427.918 kb on - strand, within BPHYT_RS01880 at 428.298 kb on + strand, within BPHYT_RS01880 at 428.417 kb on + strand, within BPHYT_RS01880 at 428.417 kb on + strand, within BPHYT_RS01880 at 428.417 kb on + strand, within BPHYT_RS01880 at 428.417 kb on + strand, within BPHYT_RS01880 at 428.418 kb on - strand, within BPHYT_RS01880 at 428.427 kb on + strand, within BPHYT_RS01880 at 428.621 kb on - strand at 428.636 kb on + strand at 428.749 kb on + strand at 428.749 kb on + strand at 428.752 kb on + strand at 428.752 kb on + strand at 428.752 kb on + strand at 428.752 kb on + strand at 428.803 kb on + strand at 428.804 kb on - strand at 428.804 kb on - strand at 428.819 kb on + strand at 428.944 kb on + strand at 428.945 kb on - strand at 428.975 kb on + strand at 428.975 kb on + strand at 428.975 kb on + strand at 428.975 kb on + strand at 429.335 kb on + strand, within BPHYT_RS01885 at 429.335 kb on + strand, within BPHYT_RS01885 at 429.335 kb on + strand, within BPHYT_RS01885 at 429.335 kb on + strand, within BPHYT_RS01885 at 429.336 kb on - strand at 429.336 kb on - strand, within BPHYT_RS01885 at 429.337 kb on + strand, within BPHYT_RS01885 at 429.461 kb on + strand, within BPHYT_RS01885 at 429.461 kb on + strand, within BPHYT_RS01885
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO9 MOI 10 remove 426,504 + +0.0 426,567 + +0.4 426,567 + +0.5 426,568 - -0.4 426,612 + BPHYT_RS01875 0.16 -1.1 426,613 - BPHYT_RS01875 0.16 -0.3 426,772 - BPHYT_RS01875 0.38 -0.9 426,772 - BPHYT_RS01875 0.38 -1.1 426,813 + BPHYT_RS01875 0.44 -0.8 426,813 + BPHYT_RS01875 0.44 -2.6 426,975 + BPHYT_RS01875 0.68 +1.0 426,975 + BPHYT_RS01875 0.68 -0.9 427,070 - BPHYT_RS01875 0.81 -1.0 427,144 - -0.5 427,908 + BPHYT_RS01880 0.41 -0.0 427,909 - BPHYT_RS01880 0.41 -0.9 427,910 + BPHYT_RS01880 0.41 +0.9 427,910 + BPHYT_RS01880 0.41 +0.2 427,910 + BPHYT_RS01880 0.41 -0.0 427,910 + BPHYT_RS01880 0.41 -0.1 427,910 + BPHYT_RS01880 0.41 +1.4 427,914 + BPHYT_RS01880 0.42 +1.6 427,914 + BPHYT_RS01880 0.42 -0.5 427,914 + BPHYT_RS01880 0.42 +0.3 427,914 + BPHYT_RS01880 0.42 -0.4 427,914 + BPHYT_RS01880 0.42 -0.0 427,914 + BPHYT_RS01880 0.42 -0.1 427,914 + BPHYT_RS01880 0.42 +2.1 427,915 - BPHYT_RS01880 0.42 +1.4 427,915 - BPHYT_RS01880 0.42 +0.7 427,915 - BPHYT_RS01880 0.42 -0.2 427,917 + BPHYT_RS01880 0.42 -1.1 427,918 - BPHYT_RS01880 0.42 +0.5 428,298 + BPHYT_RS01880 0.77 -1.4 428,417 + BPHYT_RS01880 0.87 -0.8 428,417 + BPHYT_RS01880 0.87 -0.1 428,417 + BPHYT_RS01880 0.87 -0.2 428,417 + BPHYT_RS01880 0.87 -1.7 428,418 - BPHYT_RS01880 0.88 -0.7 428,427 + BPHYT_RS01880 0.88 +0.1 428,621 - -0.5 428,636 + +0.0 428,749 + +0.2 428,749 + +1.6 428,752 + -0.1 428,752 + -0.4 428,752 + +0.3 428,752 + -3.1 428,803 + +0.5 428,804 - +0.9 428,804 - +0.4 428,819 + -0.7 428,944 + +0.9 428,945 - +1.5 428,975 + -0.1 428,975 + -0.1 428,975 + +0.3 428,975 + +0.5 429,335 + BPHYT_RS01885 0.31 +0.3 429,335 + BPHYT_RS01885 0.31 -0.3 429,335 + BPHYT_RS01885 0.31 +0.6 429,335 + BPHYT_RS01885 0.31 +0.7 429,336 - +3.7 429,336 - BPHYT_RS01885 0.31 +0.1 429,337 + BPHYT_RS01885 0.31 -2.0 429,461 + BPHYT_RS01885 0.42 +0.3 429,461 + BPHYT_RS01885 0.42 -0.6
Or see this region's nucleotide sequence