Experiment: 24hr PHYTO9 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS00255 and BPHYT_RS00260 are separated by 80 nucleotides BPHYT_RS00260 and BPHYT_RS00265 overlap by 8 nucleotides BPHYT_RS00265 and BPHYT_RS00270 overlap by 4 nucleotides BPHYT_RS00270 and BPHYT_RS00275 are separated by 37 nucleotides
BPHYT_RS00255: BPHYT_RS00255 - amino acid ABC transporter, at 65,601 to 66,764
_RS00255
BPHYT_RS00260: BPHYT_RS00260 - amino acid ABC transporter, at 66,845 to 67,681
_RS00260
BPHYT_RS00265: BPHYT_RS00265 - branched-chain amino acid ABC transporter substrate-binding protein, at 67,674 to 69,488
_RS00265
BPHYT_RS00270: BPHYT_RS00270 - branched-chain amino acid ABC transporter, at 69,485 to 70,186
_RS00270
BPHYT_RS00275: BPHYT_RS00275 - urease subunit beta, at 70,224 to 70,910
_RS00275
Position (kb) 67
68
69
70 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 66.686 kb on - strand at 66.842 kb on + strand at 66.842 kb on + strand at 66.843 kb on - strand at 66.843 kb on - strand at 66.843 kb on - strand at 66.843 kb on - strand at 66.844 kb on + strand at 66.845 kb on - strand at 66.927 kb on + strand at 66.928 kb on - strand at 66.994 kb on + strand, within BPHYT_RS00260 at 66.995 kb on - strand, within BPHYT_RS00260 at 67.094 kb on + strand, within BPHYT_RS00260 at 67.236 kb on - strand, within BPHYT_RS00260 at 67.236 kb on - strand, within BPHYT_RS00260 at 67.260 kb on + strand, within BPHYT_RS00260 at 67.261 kb on - strand, within BPHYT_RS00260 at 67.592 kb on + strand, within BPHYT_RS00260 at 67.594 kb on + strand, within BPHYT_RS00260 at 67.851 kb on + strand at 67.851 kb on + strand at 67.854 kb on - strand at 67.854 kb on - strand at 67.854 kb on - strand at 67.854 kb on - strand at 67.902 kb on + strand, within BPHYT_RS00265 at 67.904 kb on + strand, within BPHYT_RS00265 at 67.918 kb on + strand, within BPHYT_RS00265 at 67.918 kb on + strand, within BPHYT_RS00265 at 67.919 kb on - strand, within BPHYT_RS00265 at 67.923 kb on - strand, within BPHYT_RS00265 at 68.058 kb on - strand, within BPHYT_RS00265 at 68.286 kb on + strand, within BPHYT_RS00265 at 68.367 kb on + strand, within BPHYT_RS00265 at 68.377 kb on - strand, within BPHYT_RS00265 at 68.413 kb on + strand, within BPHYT_RS00265 at 68.532 kb on + strand, within BPHYT_RS00265 at 68.533 kb on - strand, within BPHYT_RS00265 at 68.798 kb on - strand, within BPHYT_RS00265 at 69.074 kb on + strand, within BPHYT_RS00265 at 69.075 kb on - strand, within BPHYT_RS00265 at 69.202 kb on + strand, within BPHYT_RS00265 at 69.203 kb on - strand, within BPHYT_RS00265 at 69.286 kb on + strand, within BPHYT_RS00265 at 69.315 kb on - strand at 69.315 kb on - strand at 69.453 kb on + strand at 69.453 kb on + strand at 69.454 kb on - strand at 69.455 kb on + strand at 69.456 kb on - strand at 69.525 kb on - strand at 69.732 kb on - strand, within BPHYT_RS00270 at 69.732 kb on - strand, within BPHYT_RS00270 at 70.094 kb on + strand, within BPHYT_RS00270 at 70.095 kb on - strand, within BPHYT_RS00270 at 70.306 kb on + strand, within BPHYT_RS00275 at 70.307 kb on - strand, within BPHYT_RS00275 at 70.460 kb on - strand, within BPHYT_RS00275 at 70.460 kb on - strand, within BPHYT_RS00275 at 70.460 kb on - strand, within BPHYT_RS00275
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO9 MOI 10 remove 66,686 - -1.2 66,842 + -0.6 66,842 + -0.3 66,843 - -0.9 66,843 - +0.3 66,843 - -0.5 66,843 - +0.2 66,844 + +0.7 66,845 - -0.5 66,927 + -0.9 66,928 - -0.8 66,994 + BPHYT_RS00260 0.18 -0.5 66,995 - BPHYT_RS00260 0.18 -1.0 67,094 + BPHYT_RS00260 0.30 -0.0 67,236 - BPHYT_RS00260 0.47 -0.6 67,236 - BPHYT_RS00260 0.47 -1.7 67,260 + BPHYT_RS00260 0.50 -0.0 67,261 - BPHYT_RS00260 0.50 -0.6 67,592 + BPHYT_RS00260 0.89 -0.7 67,594 + BPHYT_RS00260 0.89 -0.3 67,851 + -0.9 67,851 + -0.7 67,854 - -0.9 67,854 - -0.9 67,854 - -1.8 67,854 - +0.5 67,902 + BPHYT_RS00265 0.13 -1.4 67,904 + BPHYT_RS00265 0.13 +0.4 67,918 + BPHYT_RS00265 0.13 +0.5 67,918 + BPHYT_RS00265 0.13 +0.2 67,919 - BPHYT_RS00265 0.13 -0.3 67,923 - BPHYT_RS00265 0.14 -0.0 68,058 - BPHYT_RS00265 0.21 -0.0 68,286 + BPHYT_RS00265 0.34 -0.0 68,367 + BPHYT_RS00265 0.38 -0.3 68,377 - BPHYT_RS00265 0.39 +2.1 68,413 + BPHYT_RS00265 0.41 +0.6 68,532 + BPHYT_RS00265 0.47 +0.8 68,533 - BPHYT_RS00265 0.47 -1.0 68,798 - BPHYT_RS00265 0.62 +0.8 69,074 + BPHYT_RS00265 0.77 +0.5 69,075 - BPHYT_RS00265 0.77 -0.4 69,202 + BPHYT_RS00265 0.84 -1.9 69,203 - BPHYT_RS00265 0.84 +0.4 69,286 + BPHYT_RS00265 0.89 +1.0 69,315 - +0.1 69,315 - +0.6 69,453 + +0.6 69,453 + -2.2 69,454 - +0.7 69,455 + -2.3 69,456 - +0.2 69,525 - -0.4 69,732 - BPHYT_RS00270 0.35 -0.9 69,732 - BPHYT_RS00270 0.35 -2.5 70,094 + BPHYT_RS00270 0.87 -0.4 70,095 - BPHYT_RS00270 0.87 -1.6 70,306 + BPHYT_RS00275 0.12 -0.9 70,307 - BPHYT_RS00275 0.12 +0.2 70,460 - BPHYT_RS00275 0.34 +0.4 70,460 - BPHYT_RS00275 0.34 -2.8 70,460 - BPHYT_RS00275 0.34 -0.4
Or see this region's nucleotide sequence