Experiment: 24hr PHYTO8 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS35995 and BPHYT_RS36000 are separated by 242 nucleotides BPHYT_RS36000 and BPHYT_RS36005 are separated by 151 nucleotides BPHYT_RS36005 and BPHYT_RS36010 are separated by 63 nucleotides BPHYT_RS36010 and BPHYT_RS36015 are separated by 58 nucleotides BPHYT_RS36015 and BPHYT_RS36020 are separated by 181 nucleotides
BPHYT_RS35995: BPHYT_RS35995 - hypothetical protein, at 32,185 to 32,418
_RS35995
BPHYT_RS36000: BPHYT_RS36000 - hypothetical protein, at 32,661 to 32,903
_RS36000
BPHYT_RS36005: BPHYT_RS36005 - hypothetical protein, at 33,055 to 33,375
_RS36005
BPHYT_RS36010: BPHYT_RS36010 - hypothetical protein, at 33,439 to 33,915
_RS36010
BPHYT_RS36015: BPHYT_RS36015 - hypothetical protein, at 33,974 to 34,153
_RS36015
BPHYT_RS36020: BPHYT_RS36020 - hypothetical protein, at 34,335 to 34,571
_RS36020
Position (kb)
33
34 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 32.125 kb on + strand at 32.172 kb on - strand at 32.286 kb on + strand, within BPHYT_RS35995 at 32.321 kb on + strand, within BPHYT_RS35995 at 32.330 kb on + strand, within BPHYT_RS35995 at 32.330 kb on + strand, within BPHYT_RS35995 at 32.331 kb on - strand, within BPHYT_RS35995 at 32.331 kb on - strand, within BPHYT_RS35995 at 32.380 kb on + strand, within BPHYT_RS35995 at 32.487 kb on + strand at 32.488 kb on - strand at 32.501 kb on - strand at 32.814 kb on - strand, within BPHYT_RS36000 at 32.829 kb on - strand, within BPHYT_RS36000 at 32.829 kb on - strand, within BPHYT_RS36000 at 32.933 kb on - strand at 32.949 kb on + strand at 33.030 kb on + strand at 33.033 kb on + strand at 33.033 kb on + strand at 33.109 kb on + strand, within BPHYT_RS36005 at 33.110 kb on - strand, within BPHYT_RS36005 at 33.110 kb on - strand, within BPHYT_RS36005 at 33.239 kb on - strand at 33.239 kb on - strand, within BPHYT_RS36005 at 33.329 kb on - strand, within BPHYT_RS36005 at 33.358 kb on + strand at 33.359 kb on - strand at 33.359 kb on - strand at 33.560 kb on + strand, within BPHYT_RS36010 at 33.774 kb on + strand, within BPHYT_RS36010 at 33.775 kb on - strand, within BPHYT_RS36010 at 33.787 kb on + strand, within BPHYT_RS36010 at 33.787 kb on + strand, within BPHYT_RS36010 at 33.787 kb on + strand, within BPHYT_RS36010 at 33.788 kb on - strand, within BPHYT_RS36010 at 33.847 kb on + strand, within BPHYT_RS36010 at 33.902 kb on + strand at 33.924 kb on - strand at 33.924 kb on - strand at 34.153 kb on + strand at 34.154 kb on - strand at 34.273 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO8 MOI 1 remove 32,125 + -0.3 32,172 - -1.0 32,286 + BPHYT_RS35995 0.43 +0.2 32,321 + BPHYT_RS35995 0.58 +0.5 32,330 + BPHYT_RS35995 0.62 -0.5 32,330 + BPHYT_RS35995 0.62 +0.7 32,331 - BPHYT_RS35995 0.62 +0.3 32,331 - BPHYT_RS35995 0.62 -1.1 32,380 + BPHYT_RS35995 0.83 -0.6 32,487 + +2.6 32,488 - +1.0 32,501 - -0.6 32,814 - BPHYT_RS36000 0.63 +1.1 32,829 - BPHYT_RS36000 0.69 +1.2 32,829 - BPHYT_RS36000 0.69 -1.8 32,933 - +4.3 32,949 + -0.2 33,030 + +0.6 33,033 + +0.6 33,033 + +1.7 33,109 + BPHYT_RS36005 0.17 -1.4 33,110 - BPHYT_RS36005 0.17 -0.1 33,110 - BPHYT_RS36005 0.17 -0.6 33,239 - -0.7 33,239 - BPHYT_RS36005 0.57 +1.0 33,329 - BPHYT_RS36005 0.85 -1.6 33,358 + +0.7 33,359 - +0.1 33,359 - -0.5 33,560 + BPHYT_RS36010 0.25 -1.4 33,774 + BPHYT_RS36010 0.70 -1.7 33,775 - BPHYT_RS36010 0.70 +1.7 33,787 + BPHYT_RS36010 0.73 +0.7 33,787 + BPHYT_RS36010 0.73 -0.2 33,787 + BPHYT_RS36010 0.73 -0.0 33,788 - BPHYT_RS36010 0.73 -0.2 33,847 + BPHYT_RS36010 0.86 +0.6 33,902 + +1.1 33,924 - -0.7 33,924 - +0.8 34,153 + -1.2 34,154 - -1.1 34,273 - -0.3
Or see this region's nucleotide sequence