Experiment: 24hr PHYTO8 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS34530 and BPHYT_RS34535 are separated by 105 nucleotides BPHYT_RS34535 and BPHYT_RS34540 are separated by 98 nucleotides BPHYT_RS34540 and BPHYT_RS34545 are separated by 78 nucleotides
BPHYT_RS34530: BPHYT_RS34530 - LysR family transcriptional regulator, at 3,354,614 to 3,355,549
_RS34530
BPHYT_RS34535: BPHYT_RS34535 - AraC family transcriptional regulator, at 3,355,655 to 3,356,614
_RS34535
BPHYT_RS34540: BPHYT_RS34540 - short-chain dehydrogenase, at 3,356,713 to 3,357,471
_RS34540
BPHYT_RS34545: BPHYT_RS34545 - transcriptional regulator, at 3,357,550 to 3,358,296
_RS34545
Position (kb)
3355
3356
3357 Strain fitness (log2 ratio)
-1
0
1 at 3354.790 kb on - strand, within BPHYT_RS34530 at 3354.807 kb on - strand, within BPHYT_RS34530 at 3355.544 kb on + strand at 3355.605 kb on + strand at 3355.606 kb on - strand at 3355.923 kb on + strand, within BPHYT_RS34535 at 3356.102 kb on + strand, within BPHYT_RS34535 at 3356.103 kb on - strand, within BPHYT_RS34535 at 3356.513 kb on + strand, within BPHYT_RS34535 at 3356.514 kb on - strand, within BPHYT_RS34535 at 3356.514 kb on - strand, within BPHYT_RS34535 at 3356.514 kb on - strand, within BPHYT_RS34535 at 3356.617 kb on + strand at 3356.617 kb on + strand at 3356.693 kb on + strand at 3357.030 kb on + strand, within BPHYT_RS34540 at 3357.175 kb on + strand, within BPHYT_RS34540 at 3357.176 kb on - strand, within BPHYT_RS34540 at 3357.277 kb on - strand, within BPHYT_RS34540 at 3357.283 kb on - strand, within BPHYT_RS34540 at 3357.403 kb on + strand at 3357.469 kb on + strand at 3357.551 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO8 MOI 1 remove 3,354,790 - BPHYT_RS34530 0.19 +0.5 3,354,807 - BPHYT_RS34530 0.21 +1.7 3,355,544 + +0.2 3,355,605 + -0.8 3,355,606 - +1.0 3,355,923 + BPHYT_RS34535 0.28 -0.6 3,356,102 + BPHYT_RS34535 0.47 -0.8 3,356,103 - BPHYT_RS34535 0.47 -0.7 3,356,513 + BPHYT_RS34535 0.89 +0.1 3,356,514 - BPHYT_RS34535 0.89 +0.2 3,356,514 - BPHYT_RS34535 0.89 -0.8 3,356,514 - BPHYT_RS34535 0.89 -1.2 3,356,617 + +0.7 3,356,617 + -0.4 3,356,693 + -0.0 3,357,030 + BPHYT_RS34540 0.42 -0.3 3,357,175 + BPHYT_RS34540 0.61 -0.9 3,357,176 - BPHYT_RS34540 0.61 -0.8 3,357,277 - BPHYT_RS34540 0.74 +0.5 3,357,283 - BPHYT_RS34540 0.75 -1.2 3,357,403 + +1.7 3,357,469 + -0.7 3,357,551 + -0.6
Or see this region's nucleotide sequence