Experiment: 24hr PHYTO8 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS30880 and BPHYT_RS30885 are separated by 156 nucleotides BPHYT_RS30885 and BPHYT_RS30890 are separated by 52 nucleotides BPHYT_RS30890 and BPHYT_RS30895 are separated by 128 nucleotides BPHYT_RS30895 and BPHYT_RS30900 are separated by 417 nucleotides
BPHYT_RS30880: BPHYT_RS30880 - peptidase S13, at 2,482,140 to 2,483,774
_RS30880
BPHYT_RS30885: BPHYT_RS30885 - hypothetical protein, at 2,483,931 to 2,484,239
_RS30885
BPHYT_RS30890: BPHYT_RS30890 - hypothetical protein, at 2,484,292 to 2,485,062
_RS30890
BPHYT_RS30895: BPHYT_RS30895 - hypothetical protein, at 2,485,191 to 2,485,400
_RS30895
BPHYT_RS30900: BPHYT_RS30900 - transcriptional regulator, at 2,485,818 to 2,486,450
_RS30900
Position (kb)
2484
2485
2486 Strain fitness (log2 ratio)
-2
-1
0
1 at 2483.330 kb on + strand, within BPHYT_RS30880 at 2483.940 kb on + strand at 2484.040 kb on - strand, within BPHYT_RS30885 at 2484.040 kb on - strand, within BPHYT_RS30885 at 2484.140 kb on + strand, within BPHYT_RS30885 at 2484.723 kb on + strand, within BPHYT_RS30890 at 2485.600 kb on - strand at 2485.984 kb on - strand, within BPHYT_RS30900 at 2485.987 kb on - strand, within BPHYT_RS30900
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO8 MOI 1 remove 2,483,330 + BPHYT_RS30880 0.73 +1.4 2,483,940 + -1.3 2,484,040 - BPHYT_RS30885 0.35 +1.6 2,484,040 - BPHYT_RS30885 0.35 -1.8 2,484,140 + BPHYT_RS30885 0.68 -1.2 2,484,723 + BPHYT_RS30890 0.56 -0.8 2,485,600 - -0.5 2,485,984 - BPHYT_RS30900 0.26 -0.1 2,485,987 - BPHYT_RS30900 0.27 +0.4
Or see this region's nucleotide sequence