Experiment: 24hr PHYTO8 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS30495 and BPHYT_RS30500 overlap by 4 nucleotides BPHYT_RS30500 and BPHYT_RS30505 overlap by 4 nucleotides BPHYT_RS30505 and BPHYT_RS30510 are separated by 24 nucleotides
BPHYT_RS30495: BPHYT_RS30495 - oxidoreductase, at 2,400,935 to 2,402,272
_RS30495
BPHYT_RS30500: BPHYT_RS30500 - ABC transporter permease, at 2,402,269 to 2,403,177
_RS30500
BPHYT_RS30505: BPHYT_RS30505 - ABC transporter permease, at 2,403,174 to 2,403,968
_RS30505
BPHYT_RS30510: BPHYT_RS30510 - aldehyde dehydrogenase, at 2,403,993 to 2,405,420
_RS30510
Position (kb)
2403
2404 Strain fitness (log2 ratio)
-2
-1
0
1 at 2402.263 kb on - strand at 2402.453 kb on - strand, within BPHYT_RS30500 at 2402.453 kb on - strand, within BPHYT_RS30500 at 2402.660 kb on + strand, within BPHYT_RS30500 at 2402.660 kb on + strand, within BPHYT_RS30500 at 2402.674 kb on + strand, within BPHYT_RS30500 at 2402.800 kb on - strand, within BPHYT_RS30500 at 2402.800 kb on - strand, within BPHYT_RS30500 at 2403.062 kb on - strand, within BPHYT_RS30500 at 2403.253 kb on + strand at 2403.559 kb on - strand, within BPHYT_RS30505 at 2403.991 kb on - strand at 2404.546 kb on + strand, within BPHYT_RS30510
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO8 MOI 1 remove 2,402,263 - +0.5 2,402,453 - BPHYT_RS30500 0.20 +0.7 2,402,453 - BPHYT_RS30500 0.20 -0.1 2,402,660 + BPHYT_RS30500 0.43 -1.9 2,402,660 + BPHYT_RS30500 0.43 +0.2 2,402,674 + BPHYT_RS30500 0.45 +0.8 2,402,800 - BPHYT_RS30500 0.58 -1.4 2,402,800 - BPHYT_RS30500 0.58 -0.8 2,403,062 - BPHYT_RS30500 0.87 +1.3 2,403,253 + +0.2 2,403,559 - BPHYT_RS30505 0.48 -2.2 2,403,991 - +0.0 2,404,546 + BPHYT_RS30510 0.39 -0.1
Or see this region's nucleotide sequence