Experiment: 24hr PHYTO8 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS28865 and BPHYT_RS28870 are separated by 73 nucleotides BPHYT_RS28870 and BPHYT_RS28875 are separated by 180 nucleotides BPHYT_RS28875 and BPHYT_RS28880 overlap by 4 nucleotides
BPHYT_RS28865: BPHYT_RS28865 - alanine racemase, at 2,026,165 to 2,027,304
_RS28865
BPHYT_RS28870: BPHYT_RS28870 - MarR family transcriptional regulator, at 2,027,378 to 2,027,785
_RS28870
BPHYT_RS28875: BPHYT_RS28875 - 5-oxo-1,2,5-tricarboxylic-3-penten acid decarboxylase, at 2,027,966 to 2,028,739
_RS28875
BPHYT_RS28880: BPHYT_RS28880 - 2-hydroxyhepta-2,4-diene-1,7-dioate isomerase, at 2,028,736 to 2,029,494
_RS28880
Position (kb) 2027
2028 Strain fitness (log2 ratio) -1
0
1
2 at 2027.094 kb on - strand, within BPHYT_RS28865 at 2027.511 kb on - strand, within BPHYT_RS28870 at 2027.865 kb on + strand at 2027.911 kb on + strand at 2028.052 kb on - strand, within BPHYT_RS28875 at 2028.096 kb on - strand, within BPHYT_RS28875 at 2028.336 kb on - strand, within BPHYT_RS28875 at 2028.782 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO8 MOI 1 remove 2,027,094 - BPHYT_RS28865 0.81 +2.2 2,027,511 - BPHYT_RS28870 0.33 -1.4 2,027,865 + -0.5 2,027,911 + -1.0 2,028,052 - BPHYT_RS28875 0.11 -0.1 2,028,096 - BPHYT_RS28875 0.17 -0.4 2,028,336 - BPHYT_RS28875 0.48 +0.4 2,028,782 - -0.1
Or see this region's nucleotide sequence