Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS28620

Experiment: 24hr PHYTO8 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS28615 and BPHYT_RS28620 are separated by 5 nucleotidesBPHYT_RS28620 and BPHYT_RS28625 are separated by 285 nucleotides BPHYT_RS28615: BPHYT_RS28615 - 6-carboxyhexanoate--CoA ligase, at 1,973,268 to 1,975,400 _RS28615 BPHYT_RS28620: BPHYT_RS28620 - enoyl-CoA hydratase, at 1,975,406 to 1,976,188 _RS28620 BPHYT_RS28625: BPHYT_RS28625 - major facilitator transporter, at 1,976,474 to 1,977,805 _RS28625 Position (kb) 1975 1976 1977Strain fitness (log2 ratio) -2 -1 0 1at 1975.459 kb on + strandat 1975.459 kb on + strandat 1975.460 kb on - strandat 1975.731 kb on + strand, within BPHYT_RS28620at 1975.770 kb on + strand, within BPHYT_RS28620at 1975.771 kb on - strand, within BPHYT_RS28620at 1975.771 kb on - strand, within BPHYT_RS28620at 1976.195 kb on - strandat 1976.307 kb on + strandat 1976.405 kb on + strandat 1976.837 kb on + strand, within BPHYT_RS28625

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Per-strain Table

Position Strand Gene LocusTag Fraction 24hr PHYTO8 MOI 1
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1,975,459 + +1.2
1,975,459 + -0.5
1,975,460 - +0.4
1,975,731 + BPHYT_RS28620 0.42 -1.5
1,975,770 + BPHYT_RS28620 0.46 -0.4
1,975,771 - BPHYT_RS28620 0.47 +1.2
1,975,771 - BPHYT_RS28620 0.47 +0.6
1,976,195 - -0.2
1,976,307 + -0.1
1,976,405 + -0.5
1,976,837 + BPHYT_RS28625 0.27 -2.3

Or see this region's nucleotide sequence