Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS28170

Experiment: 24hr PHYTO8 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS28160 and BPHYT_RS28165 are separated by 178 nucleotidesBPHYT_RS28165 and BPHYT_RS28170 are separated by 10 nucleotidesBPHYT_RS28170 and BPHYT_RS28175 are separated by 765 nucleotides BPHYT_RS28160: BPHYT_RS28160 - MFS transporter, at 1,875,201 to 1,876,445 _RS28160 BPHYT_RS28165: BPHYT_RS28165 - histidine kinase, at 1,876,624 to 1,877,199 _RS28165 BPHYT_RS28170: BPHYT_RS28170 - nitrate ABC transporter substrate-binding protein, at 1,877,210 to 1,878,361 _RS28170 BPHYT_RS28175: BPHYT_RS28175 - iron ABC transporter substrate-binding protein, at 1,879,127 to 1,880,215 _RS28175 Position (kb) 1877 1878 1879Strain fitness (log2 ratio) -2 -1 0 1 2 3at 1876.532 kb on - strandat 1877.107 kb on + strand, within BPHYT_RS28165at 1877.658 kb on - strand, within BPHYT_RS28170at 1877.658 kb on - strand, within BPHYT_RS28170at 1877.658 kb on - strand, within BPHYT_RS28170at 1877.831 kb on - strand, within BPHYT_RS28170at 1877.831 kb on - strand, within BPHYT_RS28170at 1878.006 kb on - strand, within BPHYT_RS28170at 1878.316 kb on + strandat 1878.493 kb on + strandat 1879.291 kb on - strand, within BPHYT_RS28175

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Per-strain Table

Position Strand Gene LocusTag Fraction 24hr PHYTO8 MOI 1
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1,876,532 - +1.5
1,877,107 + BPHYT_RS28165 0.84 -0.2
1,877,658 - BPHYT_RS28170 0.39 +3.0
1,877,658 - BPHYT_RS28170 0.39 -0.9
1,877,658 - BPHYT_RS28170 0.39 -2.3
1,877,831 - BPHYT_RS28170 0.54 -1.8
1,877,831 - BPHYT_RS28170 0.54 -1.1
1,878,006 - BPHYT_RS28170 0.69 -2.2
1,878,316 + -0.1
1,878,493 + -1.7
1,879,291 - BPHYT_RS28175 0.15 -0.3

Or see this region's nucleotide sequence