Experiment: 24hr PHYTO8 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS28075 and BPHYT_RS28080 are separated by 195 nucleotides BPHYT_RS28080 and BPHYT_RS28085 are separated by 398 nucleotides BPHYT_RS28085 and BPHYT_RS28090 are separated by 70 nucleotides BPHYT_RS28090 and BPHYT_RS28095 are separated by 32 nucleotides BPHYT_RS28095 and BPHYT_RS28100 are separated by 25 nucleotides BPHYT_RS28100 and BPHYT_RS28105 are separated by 55 nucleotides
BPHYT_RS28075: BPHYT_RS28075 - cytochrome B561, at 1,862,447 to 1,862,977
_RS28075
BPHYT_RS28080: BPHYT_RS28080 - hypothetical protein, at 1,863,173 to 1,863,463
_RS28080
BPHYT_RS28085: BPHYT_RS28085 - Fis family transcriptional regulator, at 1,863,862 to 1,865,853
_RS28085
BPHYT_RS28090: BPHYT_RS28090 - ferredoxin, at 1,865,924 to 1,866,235
_RS28090
BPHYT_RS28095: BPHYT_RS28095 - membrane protein, at 1,866,268 to 1,866,462
_RS28095
BPHYT_RS28100: BPHYT_RS28100 - hypothetical protein, at 1,866,488 to 1,866,682
_RS28100
BPHYT_RS28105: BPHYT_RS28105 - hypothetical protein, at 1,866,738 to 1,867,382
_RS28105
Position (kb)
1863
1864
1865
1866 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1863.482 kb on + strand at 1863.482 kb on + strand at 1863.482 kb on + strand at 1863.771 kb on - strand at 1863.773 kb on + strand at 1863.774 kb on - strand at 1863.774 kb on - strand at 1863.829 kb on + strand at 1864.126 kb on - strand, within BPHYT_RS28085 at 1864.355 kb on + strand, within BPHYT_RS28085 at 1864.741 kb on + strand, within BPHYT_RS28085 at 1865.330 kb on - strand, within BPHYT_RS28085 at 1865.330 kb on - strand, within BPHYT_RS28085 at 1865.333 kb on - strand, within BPHYT_RS28085 at 1865.812 kb on - strand at 1865.890 kb on + strand at 1865.891 kb on - strand at 1866.104 kb on + strand, within BPHYT_RS28090 at 1866.105 kb on - strand, within BPHYT_RS28090 at 1866.153 kb on - strand, within BPHYT_RS28090 at 1866.153 kb on - strand, within BPHYT_RS28090 at 1866.575 kb on - strand, within BPHYT_RS28100 at 1866.591 kb on + strand, within BPHYT_RS28100 at 1866.648 kb on + strand, within BPHYT_RS28100 at 1866.649 kb on - strand, within BPHYT_RS28100 at 1866.773 kb on - strand at 1866.811 kb on + strand, within BPHYT_RS28105
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO8 MOI 1 remove 1,863,482 + +0.9 1,863,482 + -1.2 1,863,482 + +0.1 1,863,771 - -0.6 1,863,773 + -0.1 1,863,774 - -0.1 1,863,774 - -1.8 1,863,829 + -0.1 1,864,126 - BPHYT_RS28085 0.13 +1.3 1,864,355 + BPHYT_RS28085 0.25 +1.3 1,864,741 + BPHYT_RS28085 0.44 -0.2 1,865,330 - BPHYT_RS28085 0.74 -0.8 1,865,330 - BPHYT_RS28085 0.74 +0.5 1,865,333 - BPHYT_RS28085 0.74 -2.9 1,865,812 - +0.3 1,865,890 + +0.7 1,865,891 - -1.8 1,866,104 + BPHYT_RS28090 0.58 -0.3 1,866,105 - BPHYT_RS28090 0.58 -1.2 1,866,153 - BPHYT_RS28090 0.73 -0.3 1,866,153 - BPHYT_RS28090 0.73 +0.3 1,866,575 - BPHYT_RS28100 0.45 +0.3 1,866,591 + BPHYT_RS28100 0.53 -0.6 1,866,648 + BPHYT_RS28100 0.82 -0.3 1,866,649 - BPHYT_RS28100 0.83 +0.0 1,866,773 - +0.4 1,866,811 + BPHYT_RS28105 0.11 +0.6
Or see this region's nucleotide sequence