Experiment: 24hr PHYTO8 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS28050 and BPHYT_RS28055 overlap by 4 nucleotides BPHYT_RS28055 and BPHYT_RS28060 are separated by 129 nucleotides BPHYT_RS28060 and BPHYT_RS28065 are separated by 288 nucleotides
BPHYT_RS28050: BPHYT_RS28050 - serine protease, at 1,857,303 to 1,858,916
_RS28050
BPHYT_RS28055: BPHYT_RS28055 - membrane protein, at 1,858,913 to 1,859,686
_RS28055
BPHYT_RS28060: BPHYT_RS28060 - hypothetical protein, at 1,859,816 to 1,860,073
_RS28060
BPHYT_RS28065: BPHYT_RS28065 - hypothetical protein, at 1,860,362 to 1,860,859
_RS28065
Position (kb) 1858
1859
1860 Strain fitness (log2 ratio) -2
-1
0
1
2 at 1858.323 kb on + strand, within BPHYT_RS28050 at 1858.323 kb on + strand, within BPHYT_RS28050 at 1858.323 kb on + strand, within BPHYT_RS28050 at 1858.324 kb on - strand, within BPHYT_RS28050 at 1858.324 kb on - strand, within BPHYT_RS28050 at 1858.324 kb on - strand, within BPHYT_RS28050 at 1858.324 kb on - strand, within BPHYT_RS28050 at 1859.184 kb on - strand, within BPHYT_RS28055 at 1859.231 kb on + strand, within BPHYT_RS28055 at 1859.232 kb on - strand, within BPHYT_RS28055 at 1859.653 kb on - strand at 1859.773 kb on + strand at 1859.804 kb on + strand at 1859.866 kb on + strand, within BPHYT_RS28060 at 1859.866 kb on + strand, within BPHYT_RS28060 at 1859.867 kb on - strand, within BPHYT_RS28060 at 1859.900 kb on + strand, within BPHYT_RS28060 at 1859.900 kb on + strand, within BPHYT_RS28060 at 1859.901 kb on - strand, within BPHYT_RS28060 at 1859.901 kb on - strand, within BPHYT_RS28060 at 1860.282 kb on - strand at 1860.319 kb on + strand at 1860.319 kb on + strand at 1860.320 kb on - strand at 1860.416 kb on + strand, within BPHYT_RS28065 at 1860.417 kb on - strand, within BPHYT_RS28065
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO8 MOI 1 remove 1,858,323 + BPHYT_RS28050 0.63 -0.5 1,858,323 + BPHYT_RS28050 0.63 +2.0 1,858,323 + BPHYT_RS28050 0.63 +0.5 1,858,324 - BPHYT_RS28050 0.63 +0.9 1,858,324 - BPHYT_RS28050 0.63 +0.4 1,858,324 - BPHYT_RS28050 0.63 -0.4 1,858,324 - BPHYT_RS28050 0.63 +0.5 1,859,184 - BPHYT_RS28055 0.35 +0.1 1,859,231 + BPHYT_RS28055 0.41 -0.9 1,859,232 - BPHYT_RS28055 0.41 +0.3 1,859,653 - -0.2 1,859,773 + -0.6 1,859,804 + -0.9 1,859,866 + BPHYT_RS28060 0.19 +0.0 1,859,866 + BPHYT_RS28060 0.19 -0.4 1,859,867 - BPHYT_RS28060 0.20 +0.8 1,859,900 + BPHYT_RS28060 0.33 +0.4 1,859,900 + BPHYT_RS28060 0.33 -0.8 1,859,901 - BPHYT_RS28060 0.33 +0.3 1,859,901 - BPHYT_RS28060 0.33 -0.9 1,860,282 - +0.1 1,860,319 + +0.2 1,860,319 + -0.3 1,860,320 - -2.1 1,860,416 + BPHYT_RS28065 0.11 -1.8 1,860,417 - BPHYT_RS28065 0.11 +2.0
Or see this region's nucleotide sequence