Experiment: 24hr PHYTO8 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS25715 and BPHYT_RS25720 are separated by 354 nucleotides BPHYT_RS25720 and BPHYT_RS25725 are separated by 98 nucleotides BPHYT_RS25725 and BPHYT_RS25730 overlap by 8 nucleotides
BPHYT_RS25715: BPHYT_RS25715 - transposase, at 1,341,829 to 1,343,262
_RS25715
BPHYT_RS25720: BPHYT_RS25720 - hypothetical protein, at 1,343,617 to 1,344,117
_RS25720
BPHYT_RS25725: BPHYT_RS25725 - hypothetical protein, at 1,344,216 to 1,344,713
_RS25725
BPHYT_RS25730: BPHYT_RS25730 - hypothetical protein, at 1,344,706 to 1,347,030
_RS25730
Position (kb) 1344
1345 Strain fitness (log2 ratio) -2
-1
0
1
2 at 1343.327 kb on - strand at 1343.331 kb on - strand at 1343.347 kb on + strand at 1343.390 kb on - strand at 1343.444 kb on + strand at 1343.676 kb on - strand, within BPHYT_RS25720 at 1343.680 kb on - strand, within BPHYT_RS25720 at 1343.879 kb on + strand, within BPHYT_RS25720 at 1343.879 kb on + strand, within BPHYT_RS25720 at 1343.879 kb on + strand, within BPHYT_RS25720 at 1343.920 kb on - strand, within BPHYT_RS25720 at 1344.123 kb on - strand at 1344.123 kb on - strand at 1344.408 kb on + strand, within BPHYT_RS25725 at 1344.535 kb on + strand at 1344.800 kb on - strand at 1345.253 kb on + strand, within BPHYT_RS25730 at 1345.312 kb on + strand, within BPHYT_RS25730 at 1345.663 kb on - strand, within BPHYT_RS25730 at 1345.663 kb on - strand, within BPHYT_RS25730
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO8 MOI 1 remove 1,343,327 - -2.2 1,343,331 - +0.9 1,343,347 + -0.8 1,343,390 - -0.8 1,343,444 + -0.4 1,343,676 - BPHYT_RS25720 0.12 -2.0 1,343,680 - BPHYT_RS25720 0.13 -0.2 1,343,879 + BPHYT_RS25720 0.52 +0.7 1,343,879 + BPHYT_RS25720 0.52 -0.7 1,343,879 + BPHYT_RS25720 0.52 +0.9 1,343,920 - BPHYT_RS25720 0.60 +0.9 1,344,123 - -0.5 1,344,123 - -0.2 1,344,408 + BPHYT_RS25725 0.39 +1.9 1,344,535 + +1.5 1,344,800 - +0.8 1,345,253 + BPHYT_RS25730 0.24 +1.5 1,345,312 + BPHYT_RS25730 0.26 +0.5 1,345,663 - BPHYT_RS25730 0.41 -0.4 1,345,663 - BPHYT_RS25730 0.41 -0.7
Or see this region's nucleotide sequence