Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS24820

Experiment: 24hr PHYTO8 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS24810 and BPHYT_RS24815 are separated by 12 nucleotidesBPHYT_RS24815 and BPHYT_RS24820 overlap by 17 nucleotidesBPHYT_RS24820 and BPHYT_RS24825 are separated by 3 nucleotidesBPHYT_RS24825 and BPHYT_RS24830 overlap by 4 nucleotides BPHYT_RS24810: BPHYT_RS24810 - hypothetical protein, at 1,135,133 to 1,135,741 _RS24810 BPHYT_RS24815: BPHYT_RS24815 - cob(II)yrinic acid a,c-diamide reductase, at 1,135,754 to 1,136,413 _RS24815 BPHYT_RS24820: BPHYT_RS24820 - cobyrinic acid a,c-diamide synthase, at 1,136,397 to 1,137,695 _RS24820 BPHYT_RS24825: BPHYT_RS24825 - cob(I)alamin adenolsyltransferase/cobinamide ATP-dependent adenolsyltransferase, at 1,137,699 to 1,138,301 _RS24825 BPHYT_RS24830: BPHYT_RS24830 - cobalamin biosynthesis protein CbiG, at 1,138,298 to 1,138,786 _RS24830 Position (kb) 1136 1137 1138Strain fitness (log2 ratio) -1 0 1 2at 1135.559 kb on + strand, within BPHYT_RS24810at 1136.452 kb on + strandat 1136.452 kb on + strandat 1136.464 kb on + strandat 1136.723 kb on - strand, within BPHYT_RS24820at 1136.723 kb on - strand, within BPHYT_RS24820at 1136.919 kb on + strand, within BPHYT_RS24820at 1137.075 kb on + strand, within BPHYT_RS24820at 1137.075 kb on + strand, within BPHYT_RS24820at 1137.883 kb on + strand, within BPHYT_RS24825at 1137.883 kb on + strand, within BPHYT_RS24825at 1137.884 kb on - strand, within BPHYT_RS24825

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Per-strain Table

Position Strand Gene LocusTag Fraction 24hr PHYTO8 MOI 1
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1,135,559 + BPHYT_RS24810 0.70 +0.1
1,136,452 + +0.3
1,136,452 + -0.1
1,136,464 + -0.2
1,136,723 - BPHYT_RS24820 0.25 +2.5
1,136,723 - BPHYT_RS24820 0.25 +0.8
1,136,919 + BPHYT_RS24820 0.40 +0.4
1,137,075 + BPHYT_RS24820 0.52 +1.3
1,137,075 + BPHYT_RS24820 0.52 -1.2
1,137,883 + BPHYT_RS24825 0.31 -0.5
1,137,883 + BPHYT_RS24825 0.31 -0.5
1,137,884 - BPHYT_RS24825 0.31 +1.1

Or see this region's nucleotide sequence