Experiment: 24hr PHYTO8 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS24090 and BPHYT_RS24095 are separated by 39 nucleotides BPHYT_RS24095 and BPHYT_RS24100 are separated by 207 nucleotides BPHYT_RS24100 and BPHYT_RS24105 are separated by 298 nucleotides
BPHYT_RS24090: BPHYT_RS24090 - ubiquinol oxidase subunit II, cyanide insensitive, at 971,375 to 972,379
_RS24090
BPHYT_RS24095: BPHYT_RS24095 - membrane protein, at 972,419 to 973,831
_RS24095
BPHYT_RS24100: BPHYT_RS24100 - hypothetical protein, at 974,039 to 974,299
_RS24100
BPHYT_RS24105: BPHYT_RS24105 - chloroperoxidase, at 974,598 to 975,419
_RS24105
Position (kb) 972
973
974 Strain fitness (log2 ratio) -1
0
1
2
3 at 971.877 kb on + strand, within BPHYT_RS24090 at 972.327 kb on + strand at 972.328 kb on - strand at 972.400 kb on - strand at 973.271 kb on - strand, within BPHYT_RS24095 at 973.531 kb on - strand, within BPHYT_RS24095 at 973.680 kb on + strand, within BPHYT_RS24095 at 973.681 kb on - strand, within BPHYT_RS24095 at 973.683 kb on + strand, within BPHYT_RS24095 at 973.684 kb on - strand, within BPHYT_RS24095 at 974.298 kb on - strand at 974.314 kb on - strand at 974.394 kb on - strand at 974.582 kb on + strand at 974.582 kb on + strand at 974.582 kb on + strand at 974.814 kb on + strand, within BPHYT_RS24105
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO8 MOI 1 remove 971,877 + BPHYT_RS24090 0.50 -1.2 972,327 + -0.3 972,328 - -0.2 972,400 - +1.3 973,271 - BPHYT_RS24095 0.60 +3.0 973,531 - BPHYT_RS24095 0.79 +0.0 973,680 + BPHYT_RS24095 0.89 +0.9 973,681 - BPHYT_RS24095 0.89 +0.2 973,683 + BPHYT_RS24095 0.89 +0.7 973,684 - BPHYT_RS24095 0.90 +1.2 974,298 - -1.2 974,314 - -0.7 974,394 - -0.4 974,582 + -0.0 974,582 + +0.6 974,582 + +0.6 974,814 + BPHYT_RS24105 0.26 -1.5
Or see this region's nucleotide sequence